PMID- 22610381
OWN - NLM
STAT- MEDLINE
DCOM- 20130307
LR  - 20220410
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 32
IP  - 11
DP  - 2012 Nov
TI  - Nanoparticulate silver increases uric acid and allantoin excretion in rats, as 
      identified by metabolomics.
PG  - 929-33
LID - 10.1002/jat.2779 [doi]
AB  - Metabolomic investigation of rat urine was employed to identify mammalian 
      metabolites affected by ionic or nanoparticulate silver. Female and male Wistar 
      rats were administered silver nanoparticles (2.25, 4.5 or 9.0 mg kg(-1) body 
      weight per day) or ionic silver (silver acetate, 9.0 mg silver kg(-1) bw per day) 
      by oral gavage for 28 days. On day 18, urine was collected for 24 h and subjected 
      to metabolomics with high performance liquid chromatography-quadropole 
      time-of-flight mass spectrometry (HPLC-QTOF-MS)-based separation and detection. 
      Principal component analysis was subsequently applied to the data. Metabolomic 
      differences in urine composition were found in female rats but not in male rats. 
      Several metabolites were identified by the use of elemental composition 
      calculated from the exact mass combined with searches in the Human Metabolome 
      Database.The metabolite identities were eventually verified by co-chromatography 
      with authentic standards. Differences were found in uric acid and its degradation 
      product, allantoin. Administration of nanoparticulate silver increased both 
      metabolites, whereas ionic silver only increased allantoin. In conclusion, 
      metabolomic investigation of rat urine showed that increased levels of uric acid 
      and allantoin were associated with exposure to nanoparticulate silver.
CI  - Copyright (c) 2012 John Wiley & Sons, Ltd.
FAU - Hadrup, Niels
AU  - Hadrup N
AD  - Division of Toxicology and Risk Assessment, National Food Institute, Technical 
      University of Denmark, Morkhoj Bygade 19, DK-2860, Soborg, Denmark. 
      niels.hadrup@gmail.com.
FAU - Lam, Henrik R
AU  - Lam HR
FAU - Loeschner, Katrin
AU  - Loeschner K
FAU - Mortensen, Alicja
AU  - Mortensen A
FAU - Larsen, Erik H
AU  - Larsen EH
FAU - Frandsen, Henrik
AU  - Frandsen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120518
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Acetates)
RN  - 0 (Silver Compounds)
RN  - 19PPS85F9H (silver acetate)
RN  - 268B43MJ25 (Uric Acid)
RN  - 344S277G0Z (Allantoin)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Acetates/administration & dosage/chemistry
MH  - Allantoin/*urine
MH  - Animals
MH  - Female
MH  - Male
MH  - Metabolomics/*methods
MH  - Metal Nanoparticles/*administration & dosage/chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Silver/*administration & dosage/chemistry
MH  - Silver Compounds/administration & dosage/chemistry
MH  - Uric Acid/*urine
EDAT- 2012/05/23 06:00
MHDA- 2013/03/08 06:00
CRDT- 2012/05/22 06:00
PHST- 2012/02/02 00:00 [received]
PHST- 2012/04/18 00:00 [revised]
PHST- 2012/04/18 00:00 [accepted]
PHST- 2012/05/22 06:00 [entrez]
PHST- 2012/05/23 06:00 [pubmed]
PHST- 2013/03/08 06:00 [medline]
AID - 10.1002/jat.2779 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2012 Nov;32(11):929-33. doi: 10.1002/jat.2779. Epub 2012 May 18.