PMID- 22611311
OWN - NLM
STAT- MEDLINE
DCOM- 20120917
LR  - 20211021
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 18
IP  - 18
DP  - 2012 May 14
TI  - Agmatine induces gastric protection against ischemic injury by reducing vascular 
      permeability in rats.
PG  - 2188-96
LID - 10.3748/wjg.v18.i18.2188 [doi]
AB  - AIM: To investigate the effect of administration of agmatine (AGM) on gastric 
      protection against ischemia reperfusion (I/R) injury. METHODS: Three groups of 
      rats (6/group); sham, gastric I/R injury, and gastric I/R + AGM (100 mg/kg, i.p. 
      given 15 min prior to gastric ischemia) were recruited. Gastric injury was 
      conducted by ligating celiac artery for 30 min and reperfusion for another 30 
      min. Gastric tissues were histologically studied and immunostained with 
      angiopoietin 1 (Ang-1) and Ang-2. Vascular endothelial growth factor (VEGF) and 
      monocyte chemoattractant protein-1 (MCP-1) were measured in gastric tissue 
      homogenate. To assess whether AKt/phosphatidyl inositol-3-kinase (PI3K) mediated 
      the effect of AGM, an additional group was pretreated with Wortmannin (WM) 
      (inhibitor of Akt/PI3K, 15 mug/kg, i.p.), prior to ischemic injury and AGM 
      treatment, and examined histologically and immunostained. Another set of 
      experiments was run to study vascular permeability of the stomach using Evan's 
      blue dye. RESULTS: AGM markedly reduced Evan's blue dye extravasation (3.58 +/- 
      0.975 mug/stomach vs 1.175 +/- 0.374 mug/stomach, P < 0.05), VEGF (36.87 +/- 2.71 
      pg/100 mg protein vs 48.4 +/- 6.53 pg/100 mg protein, P < 0.05) and MCP-1 tissue 
      level (29.5 +/- 7 pg/100 mg protein vs 41.17 +/- 10.4 pg/100 mg protein, P < 0.01). 
      It preserved gastric histology and reduced congestion. Ang-1 and Ang-2 
      immunostaining were reduced in stomach sections of AGM-treated animals. The 
      administration of WM abolished the protective effects of AGM and extensive 
      hemorrhage and ulcerations were seen. CONCLUSION: AGM protects the stomach 
      against I/R injury by reducing vascular permeability and inflammation. This 
      protection is possibly mediated by Akt/PI3K.
FAU - Al Masri, Abeer A
AU  - Al Masri AA
AD  - Department of Physiology, College of Medicine and King Khalid University 
      Hospital, King Saud University, Riyadh 11461, Saudi Arabia.
FAU - El Eter, Eman
AU  - El Eter E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Androstadienes)
RN  - 0 (Angiopoietin-1)
RN  - 0 (Angiopoietin-2)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Protective Agents)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - 70J407ZL5Q (Agmatine)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Agmatine/*pharmacology
MH  - Androstadienes/pharmacology
MH  - Angiopoietin-1/metabolism
MH  - Angiopoietin-2/metabolism
MH  - Animals
MH  - Capillary Permeability/*drug effects
MH  - Chemokine CCL2/metabolism
MH  - Cytoprotection
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Gastric Mucosa/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Protective Agents/*pharmacology
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Proto-Oncogene Proteins c-akt/antagonists & inhibitors/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/metabolism/pathology/*prevention & control
MH  - Stomach/*blood supply/*drug effects/pathology
MH  - Vascular Endothelial Growth Factor A/metabolism
MH  - Wortmannin
PMC - PMC3351768
OTO - NOTNLM
OT  - Agmatine
OT  - Ischemia reperfusion injury
OT  - Monocyte chemoattractant protein-1
OT  - Stomach
OT  - Vascular endothelial growth factor
OT  - Vascular permeability
OT  - Wortmannin
EDAT- 2012/05/23 06:00
MHDA- 2012/09/18 06:00
PMCR- 2012/05/14
CRDT- 2012/05/22 06:00
PHST- 2011/11/26 00:00 [received]
PHST- 2012/01/04 00:00 [revised]
PHST- 2012/03/09 00:00 [accepted]
PHST- 2012/05/22 06:00 [entrez]
PHST- 2012/05/23 06:00 [pubmed]
PHST- 2012/09/18 06:00 [medline]
PHST- 2012/05/14 00:00 [pmc-release]
AID - 10.3748/wjg.v18.i18.2188 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2012 May 14;18(18):2188-96. doi: 10.3748/wjg.v18.i18.2188.