PMID- 22612931
OWN - NLM
STAT- MEDLINE
DCOM- 20121009
LR  - 20120522
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 95
IP  - 6
DP  - 2012 Jun
TI  - Selection of suitable reference genes for quantitative gene expression studies in 
      milk somatic cells of lactating cows (Bos indicus).
PG  - 2935-45
LID - 10.3168/jds.2011-4442 [doi]
AB  - We assessed the suitability of 9 internal control genes (ICG) in milk somatic 
      cells of lactating cows to find suitable reference genes for use in quantitative 
      PCR (qPCR). Eighteen multiparous lactating Sahiwal cows were used, 6 in each of 3 
      lactation stages: early (25 +/- 5 d in milk), mid (160 +/- 15 d in milk), and late 
      (275 +/- 25 d in milk) lactation. Nine candidate reference genes [glyceraldehyde 
      3-phosphate dehydrogenase (GAPDH), protein phosphatase 1 regulatory subunit 11 
      (PPP1R11), beta-actin (ACTB), beta-2 microglobulin (B2M), 40S ribosomal protein S15a 
      (RPS15A), ubiquitously expressed transcript (UXT), mitochondrial GTPase 1 (MTG1), 
      18S rRNA (RN18S1), and ubiquitin (UBC)] were evaluated. Three genes, beta-casein 
      (CSN2), lactoferrin (LTF), and cathelicidin (CAMP) were chosen as target genes. 
      Very high amplification was observed in 7 ICG and very low level amplification 
      was observed in 2 ICG (UXT and MTG1). Thus, UXT and MTG1 were excluded from 
      further analysis. The qPCR data were analyzed by 2 software packages, geNorm and 
      NormFinder, to determine suitable reference genes, based on their stability and 
      expression. Overall, PPP1R11, ACTB, UBC, and GAPDH were stably expressed among 
      all candidate reference genes. Therefore, these genes could be used as ICG for 
      normalization of qPCR data in milk somatic cells through lactation.
CI  - Copyright (c) 2012 American Dairy Science Association. Published by Elsevier Inc. 
      All rights reserved.
FAU - Varshney, N
AU  - Varshney N
AD  - National Dairy Research Institute, Karnal 132001, India.
FAU - Mohanty, A K
AU  - Mohanty AK
FAU - Kumar, S
AU  - Kumar S
FAU - Kaushik, J K
AU  - Kaushik JK
FAU - Dang, A K
AU  - Dang AK
FAU - Mukesh, M
AU  - Mukesh M
FAU - Mishra, B P
AU  - Mishra BP
FAU - Kataria, R
AU  - Kataria R
FAU - Kimothi, S P
AU  - Kimothi SP
FAU - Mukhopadhyay, T K
AU  - Mukhopadhyay TK
FAU - Malakar, D
AU  - Malakar D
FAU - Prakash, B S
AU  - Prakash BS
FAU - Grover, S
AU  - Grover S
FAU - Batish, V K
AU  - Batish VK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Animals
MH  - Cattle/genetics
MH  - DNA, Complementary/genetics
MH  - Female
MH  - Gene Expression/genetics
MH  - Genes/*genetics
MH  - Lactation/*genetics
MH  - Milk/*cytology
MH  - *Quantitative Trait, Heritable
MH  - Real-Time Polymerase Chain Reaction/veterinary
EDAT- 2012/05/23 06:00
MHDA- 2012/10/10 06:00
CRDT- 2012/05/23 06:00
PHST- 2011/04/13 00:00 [received]
PHST- 2012/01/16 00:00 [accepted]
PHST- 2012/05/23 06:00 [entrez]
PHST- 2012/05/23 06:00 [pubmed]
PHST- 2012/10/10 06:00 [medline]
AID - S0022-0302(12)00270-6 [pii]
AID - 10.3168/jds.2011-4442 [doi]
PST - ppublish
SO  - J Dairy Sci. 2012 Jun;95(6):2935-45. doi: 10.3168/jds.2011-4442.