PMID- 22613718 OWN - NLM STAT- MEDLINE DCOM- 20120926 LR - 20211021 IS - 1083-351X (Electronic) IS - 0021-9258 (Print) IS - 0021-9258 (Linking) VI - 287 IP - 29 DP - 2012 Jul 13 TI - Evidence that formation of vimentin mitogen-activated protein kinase (MAPK) complex mediates mast cell activation following FcepsilonRI/CC chemokine receptor 1 cross-talk. PG - 24516-24 LID - 10.1074/jbc.M111.319624 [doi] AB - Accumulating evidence points to cross-talk between FcepsilonRI and CC chemokine receptor (CCR)-mediated signaling pathways in mast cells. Here, we propose that vimentin, a protein comprising type III intermediate filament, participates in such cross-talk for CCL2/monocyte chemotactic protein 1 (MCP-1) production in mast cells, which is a mechanism for allergic inflammation. Co-stimulation via FcepsilonRI, using IgE/antigen, and CCR1, using recombinant CCL3/macrophage inflammatory protein-1alpha (MIP-1alpha), increased expression of phosphorylated, disassembled, and soluble vimentin in rat basophilic leukemia (RBL)-2H3 cells expressing human CCR1 (RBL-CCR1 cells) and bone marrow-derived murine mast cells, both models of mucosal type mast cells. Furthermore, co-stimulation enhanced production of CCL2 as well as phosphorylation of MAPK. Treating the cells with p38 MAPK inhibitor SB203580, but not with MEK inhibitor PD98058, reduced CCL2 production, suggesting that p38 MAPK, but not ERK1/2, plays a critical role in the chemokine production. Immunoprecipitation analysis showed that vimentin interacts with phosphorylated ERK1/2 and p38 MAPKs in the co-simulated cells. Preventing disassembly of the vimentin by aggregating vimentin filaments using beta,beta'-iminodipropionitrile reduced the interaction of vimentin with phosphorylated MAPKs as well as CCL2 production in the cells. Taken together, disassembled vimentin interacting with phosphorylated p38 MAPK could mediate CCL2 production in mast cells upon FcepsilonRI and CCR1 activation. FAU - Toda, Masako AU - Toda M AD - Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio 45221-0097,USA. FAU - Kuo, Chuan-Hui AU - Kuo CH FAU - Borman, Satty K AU - Borman SK FAU - Richardson, Ricardo Micheler AU - Richardson RM FAU - Inoko, Akihito AU - Inoko A FAU - Inagaki, Masaki AU - Inagaki M FAU - Collins, Andrea AU - Collins A FAU - Schneider, Klaus AU - Schneider K FAU - Ono, Santa Jeremy AU - Ono SJ LA - eng GR - AI-38910/AI/NIAID NIH HHS/United States GR - R01-EY-019630-01/EY/NEI NIH HHS/United States GR - R29 AI038910/AI/NIAID NIH HHS/United States GR - R01 EY019630/EY/NEI NIH HHS/United States GR - R01 AI038910/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20120521 PL - United States TA - J Biol Chem JT - The Journal of biological chemistry JID - 2985121R RN - 0 (Chemokine CCL2) RN - 0 (Imidazoles) RN - 0 (Organic Chemicals) RN - 0 (PD 98058) RN - 0 (Pyridines) RN - 0 (Receptors, CCR1) RN - 0 (Receptors, IgG) RN - 0 (Vimentin) RN - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1) RN - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3) RN - EC 2.7.11.24 (Mitogen-Activated Protein Kinases) RN - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) RN - OU13V1EYWQ (SB 203580) SB - IM MH - Animals MH - Cell Line MH - Cell Line, Tumor MH - Cells, Cultured MH - Chemokine CCL2/metabolism MH - Electrophoresis, Gel, Two-Dimensional MH - Enzyme-Linked Immunosorbent Assay MH - Humans MH - Imidazoles/pharmacology MH - Immunoprecipitation MH - Mast Cells/drug effects/*metabolism MH - Mice MH - Mice, Inbred BALB C MH - Mitogen-Activated Protein Kinase 1/metabolism MH - Mitogen-Activated Protein Kinase 3/metabolism MH - Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism MH - Organic Chemicals/pharmacology MH - Phosphorylation MH - Protein Binding MH - Pyridines/pharmacology MH - Rats MH - Receptors, CCR1/*metabolism MH - Receptors, IgG/*metabolism MH - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MH - Vimentin/*metabolism MH - p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism PMC - PMC3397876 EDAT- 2012/05/23 06:00 MHDA- 2012/09/27 06:00 PMCR- 2013/07/13 CRDT- 2012/05/23 06:00 PHST- 2012/05/23 06:00 [entrez] PHST- 2012/05/23 06:00 [pubmed] PHST- 2012/09/27 06:00 [medline] PHST- 2013/07/13 00:00 [pmc-release] AID - S0021-9258(20)43300-9 [pii] AID - M111.319624 [pii] AID - 10.1074/jbc.M111.319624 [doi] PST - ppublish SO - J Biol Chem. 2012 Jul 13;287(29):24516-24. doi: 10.1074/jbc.M111.319624. Epub 2012 May 21.