PMID- 22613963
OWN - NLM
STAT- MEDLINE
DCOM- 20121016
LR  - 20211203
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 6
IP  - 2
DP  - 2012 Aug
TI  - Protective effect of transplanted bone marrow-derived mesenchymal stem cells on 
      pancreatitis-associated lung injury in rats.
PG  - 287-92
LID - 10.3892/mmr.2012.922 [doi]
AB  - Severe acute pancreatitis (SAP) is initiated by the premature activation of 
      digestive enzymes within the pancreatic acinar cells, leading to self-digestion 
      and inflammatory responses in pancreatic ductal cells, thus giving rise to 
      systemic inflammatory response syndrome (SIRS). The most common and serious SIRS 
      is pancreatitis-associated lung injury, and inflammatory mediators play an 
      important role in its pathogenesis. Bone marrow-derived mesenchymal stem cells 
      (MSCs) are differentiated into alveolar endothelial cells to replace the damaged 
      alveolar endothelial cells and inhibit inflammatory response in the injured lung 
      tissues. In this study, we aimed to investigate the therapeutic effect of bone 
      marrow-derived MSCs in rats with pancreatitis-associated lung injury. 
      Experimental SAP was induced by a retrograde injection of 5% sodium taurocholate 
      into the biliopancreatic duct of 75 male Sprague-Dawley rats, which were divided 
      into the SAP group (n=25), the MSC group (n=25) and the sham-operated group 
      (n=25) to explore the pathology and function of lung tissues and the regulation 
      of inflammatory mediators. Pulmonary edema was estimated by measuring water 
      content in the lung tissues. Pulmonary myeloperoxidase (MPO) activity was 
      detected using spectrophotometry. Serum amylase was detected using the Automatic 
      Biochemistry Analyzer. Tumor necrosis factor-alpha (TNF-alpha) and substance P (SP) mRNA 
      levels were determined by quantitative reverse transcriptase-polymerase chain 
      reaction. Our results showed that serum amylase activity was significantly 
      decreased in the MSC group compared to the SAP group. Pulmonary edema was 
      significantly diminished (p<0.05) in the MSC group compared to the SAP group. 
      Typical acute lung injury was observed in the SAP group, and the pathological 
      changes were mild in the MSC group. The expression of TNF-alpha and SP mRNA in lung 
      tissue was diminished in the MSC group compared to the SAP group. In conclusion, 
      MSC transplantation attenuates pulmonary edema and inflammation, and reduces the 
      mRNA expression of TNF-alpha and SP in pancreatitis-associated lung injury.
FAU - Wang, Lie
AU  - Wang L
AD  - Department of General Surgery, Fuzhou General Hospital of Nanjing Military 
      Region, Fuzhou, Fujian 350025, PR China.
FAU - Tu, Xiao-Huang
AU  - Tu XH
FAU - Zhao, Peng
AU  - Zhao P
FAU - Song, Jing-Xiang
AU  - Song JX
FAU - Zou, Zhong-Dong
AU  - Zou ZD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120521
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Inflammation Mediators)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 33507-63-0 (Substance P)
RN  - 5E090O0G3Z (Taurocholic Acid)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 3.2.1.- (Amylases)
SB  - IM
MH  - Acute Lung Injury/enzymology/metabolism/pathology/*therapy
MH  - Amylases/blood
MH  - Animals
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Disease Models, Animal
MH  - Enzyme Activation
MH  - Inflammation Mediators/blood
MH  - Lung/enzymology/metabolism/pathology
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Pancreatitis/chemically induced/metabolism/*pathology
MH  - Peroxidase/metabolism
MH  - Pulmonary Edema/pathology/therapy
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Severity of Illness Index
MH  - Substance P/blood
MH  - Systemic Inflammatory Response Syndrome/chemically induced/therapy
MH  - Taurocholic Acid/administration & dosage/adverse effects
MH  - Tumor Necrosis Factor-alpha/blood
OTO - NOTNLM
OT  - mesenchymal stem cells
OT  - severe acute pancreatitis
OT  - pancreatitis-associated lung injury
OT  - substance P
OT  - tumor necrosis factor-alpha
EDAT- 2012/05/23 06:00
MHDA- 2012/10/17 06:00
CRDT- 2012/05/23 06:00
PHST- 2012/01/18 00:00 [received]
PHST- 2012/04/10 00:00 [accepted]
PHST- 2012/05/23 06:00 [entrez]
PHST- 2012/05/23 06:00 [pubmed]
PHST- 2012/10/17 06:00 [medline]
AID - 10.3892/mmr.2012.922 [doi]
PST - ppublish
SO  - Mol Med Rep. 2012 Aug;6(2):287-92. doi: 10.3892/mmr.2012.922. Epub 2012 May 21.