PMID- 22615205
OWN - NLM
STAT- MEDLINE
DCOM- 20120907
LR  - 20151119
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 189
IP  - 1
DP  - 2012 Jul 1
TI  - Dendritic cell-specific transcript: dendritic cell marker and regulator of 
      TLR-induced cytokine production.
PG  - 138-45
LID - 10.4049/jimmunol.1103709 [doi]
AB  - Dendritic cells (DCs) are the professional APCs of the immune system that dictate 
      the type and course of an immune response. Molecular understanding of DC biology 
      is important for the design of DC-based immunotherapies and optimal clinical 
      applications in vaccination settings. Previously, we isolated and characterized 
      the cDNA-encoding dendritic cell-specific transcript (DC-SCRIPT; also known as 
      ZNF366). DC-SCRIPT mRNA expression in the immune system was confined to DCs and 
      was reported to be an early hallmark of DC differentiation. In this study, we 
      demonstrate IL-4 to be the dominant factor for DC-SCRIPT expression in human 
      monocyte-derived DCs. In addition, to our knowledge, we show for the first time 
      endogenous DC-SCRIPT protein expression in human DCs both in vitro and in situ. 
      DC-SCRIPT protein is detected early upon differentiation of monocytes into DCs 
      and is also present in multiple freshly isolated DC subsets. Maturation of DCs 
      with TLR ligands further increased DC-SCRIPT mRNA expression, suggesting a role 
      in DC maturation. Indeed, small interfering RNA-mediated knockdown of DC-SCRIPT 
      affected the cytokine response upon TLR stimulation. These DCs displayed enhanced 
      IL-10 and decreased IL-12 production, compared with wild-type DCs. Silencing of 
      IL-10 in DC-SCRIPT knockdown DCs rescued IL-12 expression, suggesting a primary 
      role for DC-SCRIPT in the regulation of IL-10 production.
FAU - Hontelez, Saartje
AU  - Hontelez S
AD  - Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, 
      Radboud University Nijmegen Medical Centre, 6525 GA Nijmegen, The Netherlands.
FAU - Ansems, Marleen
AU  - Ansems M
FAU - Karthaus, Nina
AU  - Karthaus N
FAU - Zuidscherwoude, Malou
AU  - Zuidscherwoude M
FAU - Looman, Maaike W
AU  - Looman MW
FAU - Triantis, Vassilis
AU  - Triantis V
FAU - Adema, Gosse J
AU  - Adema GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120521
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Biomarkers)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytokines)
RN  - 0 (IL10 protein, human)
RN  - 0 (IL4 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (ZNF366 protein, human)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Carrier Proteins/biosynthesis/genetics/*physiology
MH  - Cells, Cultured
MH  - Cytokines/*biosynthesis/genetics
MH  - Dendritic Cells/*immunology/metabolism
MH  - Gene Expression Regulation/immunology
MH  - Humans
MH  - Interleukin-10/biosynthesis
MH  - Interleukin-4/physiology
MH  - Molecular Dynamics Simulation
MH  - Monocytes/immunology/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Toll-Like Receptors/*physiology
EDAT- 2012/05/23 06:00
MHDA- 2012/09/08 06:00
CRDT- 2012/05/23 06:00
PHST- 2012/05/23 06:00 [entrez]
PHST- 2012/05/23 06:00 [pubmed]
PHST- 2012/09/08 06:00 [medline]
AID - jimmunol.1103709 [pii]
AID - 10.4049/jimmunol.1103709 [doi]
PST - ppublish
SO  - J Immunol. 2012 Jul 1;189(1):138-45. doi: 10.4049/jimmunol.1103709. Epub 2012 May 
      21.