PMID- 22619482
OWN - NLM
STAT- MEDLINE
DCOM- 20120920
LR  - 20211021
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2012
DP  - 2012
TI  - oxLDL downregulates the dendritic cell homing factors CCR7 and CCL21.
PG  - 320953
LID - 10.1155/2012/320953 [doi]
LID - 320953
AB  - INTRODUCTION: Dendritic cells (DCs) and oxLDL play an important role in the 
      atherosclerotic process with DCs accumulating in the plaques during plaque 
      progression. Our aim was to investigate the role of oxLDL in the modulation of 
      the DC homing-receptor CCR7 and endothelial-ligand CCL21. METHODS AND RESULTS: 
      The expression of the DC homing-receptor CCR7 and its endothelial-ligand CCL21 
      was examined on atherosclerotic carotic plaques of 47 patients via qRT-PCR and 
      immunofluorescence. In vitro, we studied the expression of CCR7 on DCs and CCL21 
      on human microvascular endothelial cells (HMECs) in response to oxLDL. CCL21- and 
      CCR7-mRNA levels were significantly downregulated in atherosclerotic plaques 
      versus non-atherosclerotic controls [90% for CCL21 and 81% for CCR7 (P < 0.01)]. 
      In vitro, oxLDL reduced CCR7 mRNA levels on DCs by 30% and protein levels by 46%. 
      Furthermore, mRNA expression of CCL21 was significantly reduced by 50% (P < 0.05) 
      and protein expression by 24% in HMECs by oxLDL (P < 0.05). CONCLUSIONS: The 
      accumulation of DCs in atherosclerotic plaques appears to be related to a 
      downregulation of chemokines and their ligands, which are known to regulate DC 
      migration. oxLDL induces an in vitro downregulation of CCR7 and CCL21, which may 
      play a role in the reduction of DC migration from the plaques.
FAU - Nickel, Thomas
AU  - Nickel T
AD  - Medizinische Klinik und Poliklinik I, Campus Grosshadern, Ludwig-Maximilians 
      University, 81377 Munich, Germany. tsnickel@web.de
FAU - Pfeiler, Susanne
AU  - Pfeiler S
FAU - Summo, Claudia
AU  - Summo C
FAU - Kopp, Reinhard
AU  - Kopp R
FAU - Meimarakis, Georgios
AU  - Meimarakis G
FAU - Sicic, Zeljka
AU  - Sicic Z
FAU - Lambert, Marius
AU  - Lambert M
FAU - Lackermair, Korbinian
AU  - Lackermair K
FAU - David, Robert
AU  - David R
FAU - Beiras-Fernandez, Andres
AU  - Beiras-Fernandez A
FAU - Kaczmarek, Ingo
AU  - Kaczmarek I
FAU - Weis, Michael
AU  - Weis M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120430
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (CCL19 protein, human)
RN  - 0 (CCL21 protein, human)
RN  - 0 (CCR7 protein, human)
RN  - 0 (Chemokine CCL19)
RN  - 0 (Chemokine CCL21)
RN  - 0 (Ligands)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Receptors, CCR7)
RN  - 0 (oxidized low density lipoprotein)
SB  - IM
MH  - Atherosclerosis/pathology
MH  - Carotid Arteries/pathology
MH  - Carotid Stenosis/pathology
MH  - Cell Movement
MH  - Chemokine CCL19/metabolism
MH  - Chemokine CCL21/*metabolism
MH  - Dendritic Cells/*cytology
MH  - Disease Progression
MH  - *Down-Regulation
MH  - Endothelial Cells/cytology/metabolism
MH  - Humans
MH  - Ligands
MH  - Lipoproteins, LDL/*metabolism
MH  - Microcirculation
MH  - Microscopy, Fluorescence/methods
MH  - Monocytes/cytology
MH  - Receptors, CCR7/*metabolism
PMC - PMC3350855
EDAT- 2012/05/24 06:00
MHDA- 2012/09/21 06:00
PMCR- 2012/04/30
CRDT- 2012/05/24 06:00
PHST- 2011/08/28 00:00 [received]
PHST- 2012/02/14 00:00 [accepted]
PHST- 2012/05/24 06:00 [entrez]
PHST- 2012/05/24 06:00 [pubmed]
PHST- 2012/09/21 06:00 [medline]
PHST- 2012/04/30 00:00 [pmc-release]
AID - 10.1155/2012/320953 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2012;2012:320953. doi: 10.1155/2012/320953. Epub 2012 Apr 30.