PMID- 22623274
OWN - NLM
STAT- MEDLINE
DCOM- 20131030
LR  - 20211021
IS  - 1437-160X (Electronic)
IS  - 0172-8172 (Linking)
VI  - 33
IP  - 4
DP  - 2013 Apr
TI  - HLA-DRbeta1*04 typing by simple in-house PCR-SSP technique for rheumatoid arthritis 
      patients.
PG  - 867-70
LID - 10.1007/s00296-012-2448-7 [doi]
AB  - A strong association between rheumatoid arthritis (RA) and human leukocyte 
      antigen (HLA) has been observed in many different populations and that accounts 
      for approximately one-third of the genetic component of RA susceptibility. The 
      greatest effect comes from DRbeta1 gene where the strongest association has been 
      found with DRbeta1*04 (DR4) allele. As serology has some disadvantages over 
      polymerase chain reaction (PCR)-based techniques and commercially available 
      PCR-based kits are expensive, this study was aimed to standardize simple in-house 
      PCR-SSP technique. Accuracy of this test was further checked with standard 
      PCR-SSOP (RLS) results. The frequency HLA-DRbeta1*04 was significantly increased 
      among RA patients when compared with normal controls. In this study, a very 
      simple, convenient and more cost-effective in-house PCR-SSP technique was 
      standardized for HLA-DRbeta1*04 typing that is helpful to RA diagnosis in developing 
      countries like India, which can be used as a good screening test.
FAU - Parasannanavar, Devaraj Jatteppa
AU  - Parasannanavar DJ
AD  - National Institute of Immunohaematology ICMR, Mumbai, Maharashtra, India.
FAU - Yeola, Avani
AU  - Yeola A
FAU - Pradhan, Vandana
AU  - Pradhan V
FAU - Rajyadhaksha, Anjali
AU  - Rajyadhaksha A
FAU - Ghosh, Kanjaksha
AU  - Ghosh K
LA  - eng
PT  - Journal Article
DEP - 20120524
PL  - Germany
TA  - Rheumatol Int
JT  - Rheumatology international
JID - 8206885
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB1*04 antigen)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Arthritis, Rheumatoid/*genetics/immunology
MH  - Female
MH  - Genotype
MH  - HLA-DRB1 Chains/*genetics
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - India
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/methods
MH  - Sensitivity and Specificity
EDAT- 2012/05/25 06:00
MHDA- 2013/10/31 06:00
CRDT- 2012/05/25 06:00
PHST- 2011/11/17 00:00 [received]
PHST- 2012/05/06 00:00 [accepted]
PHST- 2012/05/25 06:00 [entrez]
PHST- 2012/05/25 06:00 [pubmed]
PHST- 2013/10/31 06:00 [medline]
AID - 10.1007/s00296-012-2448-7 [doi]
PST - ppublish
SO  - Rheumatol Int. 2013 Apr;33(4):867-70. doi: 10.1007/s00296-012-2448-7. Epub 2012 
      May 24.