PMID- 22623774
OWN - NLM
STAT- MEDLINE
DCOM- 20121121
LR  - 20211021
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 86
IP  - 15
DP  - 2012 Aug
TI  - A novel bat herpesvirus encodes homologues of major histocompatibility complex 
      classes I and II, C-type lectin, and a unique family of immune-related genes.
PG  - 8014-30
LID - 10.1128/JVI.00723-12 [doi]
AB  - Herpesviruses or herpesviral sequences have been identified in various bat 
      species. Here, we report the isolation, cell tropism, and complete genome 
      sequence of a novel betaherpesvirus from the bat Miniopterus schreibersii (MsHV). 
      In primary cell culture, MsHV causes cytopathic effects (CPE) and reaches peak 
      virus production 2 weeks after infection. MsHV was found to infect and replicate 
      less efficiently in a feline kidney cell, CRFK, and failed to replicate in 13 
      other cell lines tested. Sequencing of the MsHV genome using the 454 system, with 
      a 224-fold coverage, revealed a genome size of 222,870 bp. The genome was 
      extensively analyzed in comparison to those of related viruses. Of the 190 
      predicted open reading frames (ORFs), 40 were identified as herpesvirus core 
      genes. Among 93 proteins with identifiable homologues in tree shrew herpesvirus 
      (THV), human cytomegalovirus (HCMV), or rat cytomegalovirus (RCMV), most had 
      highest sequence identities with THV counterparts. However, the MsHV genome 
      organization is colinear with that of RCMV rather than that of THV. The following 
      unique features were discovered in the MsHV genome. One predicted protein, B125, 
      is similar to human herpesvirus 6 (HHV-6) U94, a homologue of the parvovirus Rep 
      protein. For the unique ORFs, 7 are predicted to encode major histocompatibility 
      complex (MHC)-related proteins, 2 to encode MHC class I homologues, and 3 to 
      encode MHC class II homologues; 4 encode the homologues of C-type lectin- or 
      natural killer cell lectin-like receptors;, and the products of a unique gene 
      family, the b149 family, of 16 members, have no significant sequence identity 
      with known proteins but exhibit immunoglobulin-like beta-sandwich domains 
      revealed by three-dimensional (3D) structural prediction. To our knowledge, MsHV 
      is the first virus genome known to encode MHC class II homologues.
FAU - Zhang, Huajun
AU  - Zhang H
AD  - CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, 
      Victoria, Australia.
FAU - Todd, Shawn
AU  - Todd S
FAU - Tachedjian, Mary
AU  - Tachedjian M
FAU - Barr, Jennifer A
AU  - Barr JA
FAU - Luo, Minhua
AU  - Luo M
FAU - Yu, Meng
AU  - Yu M
FAU - Marsh, Glenn A
AU  - Marsh GA
FAU - Crameri, Gary
AU  - Crameri G
FAU - Wang, Lin-Fa
AU  - Wang LF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120523
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Cats
MH  - Cell Line
MH  - Chiroptera
MH  - *Genome, Viral
MH  - Herpesviridae/*genetics/metabolism
MH  - Histocompatibility Antigens Class I/*genetics/metabolism
MH  - Histocompatibility Antigens Class II/*genetics/metabolism
MH  - Humans
MH  - Lectins, C-Type/*genetics/metabolism
MH  - Open Reading Frames/*genetics
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Viral Proteins/*genetics/metabolism
PMC - PMC3421651
EDAT- 2012/05/25 06:00
MHDA- 2012/12/10 06:00
PMCR- 2013/02/01
CRDT- 2012/05/25 06:00
PHST- 2012/05/25 06:00 [entrez]
PHST- 2012/05/25 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
PHST- 2013/02/01 00:00 [pmc-release]
AID - JVI.00723-12 [pii]
AID - 00723-12 [pii]
AID - 10.1128/JVI.00723-12 [doi]
PST - ppublish
SO  - J Virol. 2012 Aug;86(15):8014-30. doi: 10.1128/JVI.00723-12. Epub 2012 May 23.