PMID- 2262426
OWN - NLM
STAT- MEDLINE
DCOM- 19910207
LR  - 20190510
IS  - 0021-8812 (Print)
IS  - 0021-8812 (Linking)
VI  - 68
IP  - 11
DP  - 1990 Nov
TI  - Active immunization of beef heifers against luteinizing hormone: III. Evaluation 
      of dose and longevity.
PG  - 3755-64
AB  - Objectives were to evaluate the dose (Exp. 1) and purity of LH preparations (Exp. 
      2) on the anti-LH antibody response in heifers. Experiment 3 evaluated the 
      longevity of LH immunization on sterility in heifers. In Exp. 1, 115 crossbred 
      heifers were injected every 3 wk for 6 wk with .1, .33, 1.0, 3.0 or 9.0 mg of 
      LH-ovalbumin. Concentrations of anti-LH antibodies generated were quantified by 
      determining the percentage of binding of [125I]LH in serum. Mena LH binding over 
      wk 0 to 12 was greater in heifers immunized with 1.0 mg conjugate than in heifers 
      immunized with other doses (P less than .05). In Exp. 2, LH-ovalbumin conjugates 
      were made from either LH-1, LH-2 or LH-3, which had relative immunological 
      potencies of 2.1, 1.5 and 1.2 x NIH-LH-S1 units/mg, respectively. Forty-eight 
      crossbred beef heifers were immunized against one of these three LH-ovalbumin 
      conjugates, against LH conjugated without ovalbumin (LH-LH), or against ovalbumin 
      alone (Oval). Estrous cycle activity was monitored by measuring serum 
      progesterone concentration. Potency of the LH preparation used in the 
      LH-ovalbumin conjugate was correlated (r = .94) with its ability to produce LH 
      antibodies. In Exp.3, heifers were injected with 1 mg antigen every 2 wk for 10 
      wk. Five LH-1 heifers and five control heifers were slaughtered for examination 
      of ovaries 10 wk after the last booster injection. The remaining five LH-I and 
      five control animals were placed with a bull 8 wk after the last booster. All 
      five control heifers conceived by 4 +/- 1 wk after placement with the bull 
      whereas the LH-immunized heifers remained acyclic for 42 to 96 wk.
FAU - Grieger, D M
AU  - Grieger DM
AD  - Washington State University, Pullman 99163.
FAU - Scarborough, R
AU  - Scarborough R
FAU - deAvila, D M
AU  - deAvila DM
FAU - Johnson, H E
AU  - Johnson HE
FAU - Reeves, J J
AU  - Reeves JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - Cattle/*immunology
MH  - Dose-Response Relationship, Immunologic
MH  - Estrus/immunology
MH  - Female
MH  - Immunization/*veterinary
MH  - Luteinizing Hormone/administration & dosage/*immunology
MH  - Ovalbumin/administration & dosage/*immunology
MH  - Progesterone/blood
MH  - Random Allocation
MH  - Sterilization, Reproductive/*veterinary
EDAT- 1990/11/01 00:00
MHDA- 1990/11/01 00:01
CRDT- 1990/11/01 00:00
PHST- 1990/11/01 00:00 [pubmed]
PHST- 1990/11/01 00:01 [medline]
PHST- 1990/11/01 00:00 [entrez]
AID - 10.2527/1990.68113755x [doi]
PST - ppublish
SO  - J Anim Sci. 1990 Nov;68(11):3755-64. doi: 10.2527/1990.68113755x.