PMID- 22626558
OWN - NLM
STAT- MEDLINE
DCOM- 20130110
LR  - 20211021
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 178
IP  - 1
DP  - 2012 Nov
TI  - Bile salts increase epithelial cell proliferation through HuR-induced c-Myc 
      expression.
PG  - 155-64
LID - S0022-4804(12)00131-X [pii]
LID - 10.1016/j.jss.2012.02.029 [doi]
AB  - BACKGROUND: Bile salts increase intestinal mucosal proliferation through an 
      increase in c-Myc, a transcription factor that controls the expression of 
      numerous translation regulatory proteins. HuR is an RNA-binding protein that 
      regulates translation of target mRNAs. RNA-binding proteins can control mRNA 
      stability by binding to AU- and U-rich elements located in the 3'-untranslated 
      regions (3'-UTRs) of target mRNAs. AIM: To determine how bile salt-induced c-Myc 
      stimulates enterocyte proliferation. METHODS: Enterocyte proliferation was 
      measured both in vivo using C57Bl6 mice and in vitro using IEC-6 cells after 
      taurodeoxycholate (TDCA) supplementation. HuR and c-Myc protein expression was 
      determined by immunoblot. c-Myc mRNA expression was determined by PCR. HuR 
      expression was inhibited using specific small interfering RNA. HuR binding to 
      c-Myc mRNA was determined by immunoprecipitation. RESULTS: TDCA increased 
      enterocyte proliferation in vivo and in vitro. TDCA stimulates translocation of 
      HuR from the nucleus to the cytoplasm. Cytoplasmic HuR regulates c-Myc 
      translation by HuR binding to the 3'-UTR of c-Myc mRNA. Increased TDCA-induced 
      c-Myc increases enterocyte proliferation. CONCLUSIONS: Bile salts have beneficial 
      effects on the intestinal epithelial mucosa, which are important in maintaining 
      intestinal mucosal integrity and function. These data further support an 
      important beneficial role of bile salts in regulation of mucosal growth and 
      repair. Decreased enterocyte exposure to luminal bile salts, as occurs during 
      critical illness, liver failure, starvation, and intestinal injury, may have a 
      detrimental effect on mucosal integrity.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Perrone, Erin E
AU  - Perrone EE
AD  - Department of Pediatric Surgery, University of Maryland School of Medicine, 
      Baltimore, Maryland 21201, USA.
FAU - Liu, Lan
AU  - Liu L
FAU - Turner, Douglas J
AU  - Turner DJ
FAU - Strauch, Eric D
AU  - Strauch ED
LA  - eng
GR  - R21 DK075409/DK/NIDDK NIH HHS/United States
PT  - Journal Article
DEP - 20120510
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Bile Acids and Salts)
RN  - 0 (ELAV Proteins)
RN  - 0 (Myc protein, mouse)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 516-50-7 (Taurodeoxycholic Acid)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Animals
MH  - Bile Acids and Salts/metabolism/*pharmacology
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cytoplasm/metabolism
MH  - ELAV Proteins/genetics/*metabolism
MH  - Enterocytes/cytology/drug effects/metabolism
MH  - Epithelial Cells/cytology/drug effects/metabolism
MH  - Gene Expression/drug effects/physiology
MH  - Intestinal Mucosa/*cytology/drug effects/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Proto-Oncogene Proteins c-myc/*genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - RNA, Small Interfering/genetics
MH  - Taurodeoxycholic Acid/metabolism/pharmacology
PMC - PMC3593244
MID - NIHMS372873
EDAT- 2012/05/26 06:00
MHDA- 2013/01/11 06:00
PMCR- 2013/11/01
CRDT- 2012/05/26 06:00
PHST- 2011/04/05 00:00 [received]
PHST- 2012/01/05 00:00 [revised]
PHST- 2012/02/16 00:00 [accepted]
PHST- 2012/05/26 06:00 [entrez]
PHST- 2012/05/26 06:00 [pubmed]
PHST- 2013/01/11 06:00 [medline]
PHST- 2013/11/01 00:00 [pmc-release]
AID - S0022-4804(12)00131-X [pii]
AID - 10.1016/j.jss.2012.02.029 [doi]
PST - ppublish
SO  - J Surg Res. 2012 Nov;178(1):155-64. doi: 10.1016/j.jss.2012.02.029. Epub 2012 May 
      10.