PMID- 22627909
OWN - NLM
STAT- MEDLINE
DCOM- 20121101
LR  - 20120625
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 32
IP  - 8
DP  - 2012 Aug
TI  - Further experience with the local lymph node assay using standard radioactive and 
      nonradioactive cell count measurements.
PG  - 597-607
LID - 10.1002/jat.2754 [doi]
AB  - In a previous study, the predictive capacity of a modified local lymph node assay 
      (LLNA) based on cell counts, the LNCC, was demonstrated to be closely similar to 
      that of the original assay. In addition, a range of substances, including some 
      technical/commercial materials and a range of agrochemical formulations (n = 180) 
      have also been assessed in both methods in parallel. The results in the LNCC and 
      LLNA were generally consistent, with 86% yielding an identical classification 
      outcome. Discordant results were associated with borderline data and were evenly 
      distributed between the two methods. Potency information derived from each method 
      also demonstrated good consistency (n = 101), with 93% of predictions being 
      close. Skin irritation was observed only infrequently and was most commonly 
      associated with positive results; it was not associated with the discordant 
      results. Where different vehicles were used with the same test material, the 
      effect on sensitizing activity was modest, consistent with historical data. 
      Analysis of positive control data indicated that the LNCC and LLNA displayed 
      similar levels of biological variation. When taken in combination with the 
      previously published results on LLNA Performance Standard chemicals, it is 
      concluded that the LNCC provides a viable non-radioactive alternative to the LLNA 
      for the assessment of substances, including potency predictions, as well as for 
      the evaluation of preparations.
CI  - Copyright (c) 2012 John Wiley & Sons, Ltd.
FAU - Kolle, Susanne N
AU  - Kolle SN
AD  - BASF SE, Experimental Toxicology and Ecology, Ludwigshafen, Germany.
FAU - Basketter, David
AU  - Basketter D
FAU - Schrage, Arnhild
AU  - Schrage A
FAU - Gamer, Armin O
AU  - Gamer AO
FAU - van Ravenzwaay, Bennard
AU  - van Ravenzwaay B
FAU - Landsiedel, Robert
AU  - Landsiedel R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20120525
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
SB  - IM
MH  - Animals
MH  - Biological Assay/*methods/*standards
MH  - Cell Count/standards
MH  - Dermatitis, Allergic Contact/metabolism/*pathology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Radiation
MH  - Female
MH  - Linear Models
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred CBA
EDAT- 2012/05/26 06:00
MHDA- 2012/11/02 06:00
CRDT- 2012/05/26 06:00
PHST- 2012/01/26 00:00 [received]
PHST- 2012/02/27 00:00 [revised]
PHST- 2012/02/27 00:00 [accepted]
PHST- 2012/05/26 06:00 [entrez]
PHST- 2012/05/26 06:00 [pubmed]
PHST- 2012/11/02 06:00 [medline]
AID - 10.1002/jat.2754 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2012 Aug;32(8):597-607. doi: 10.1002/jat.2754. Epub 2012 May 25.