PMID- 22628364
OWN - NLM
STAT- MEDLINE
DCOM- 20120917
LR  - 20220310
IS  - 1095-8290 (Electronic)
IS  - 0305-7364 (Print)
IS  - 0305-7364 (Linking)
VI  - 109
IP  - 7
DP  - 2012 Jun
TI  - Seed colour loci, homoeology and linkage groups of the C genome chromosomes 
      revealed in Brassica rapa-B. oleracea monosomic alien addition lines.
PG  - 1227-42
LID - 10.1093/aob/mcs052 [doi]
AB  - BACKGROUND AND AIMS: Brassica rapa and B. oleracea are the progenitors of oilseed 
      rape B. napus. The addition of each chromosome of B. oleracea to the chromosome 
      complement of B. rapa results in a series of monosomic alien addition lines 
      (MAALs). Analysis of MAALs determines which B. oleracea chromosomes carry genes 
      controlling specific phenotypic traits, such as seed colour. Yellow-seeded 
      oilseed rape is a desirable breeding goal both for food and livestock feed 
      end-uses that relate to oil, protein and fibre contents. The aims of this study 
      included developing a missing MAAL to complement an available series, for studies 
      on seed colour control, chromosome homoeology and assignment of linkage groups to 
      B. oleracea chromosomes. METHODS: A new batch of B. rapa-B. oleracea aneuploids 
      was produced to generate the missing MAAL. Seed colour and other plant 
      morphological features relevant to differentiation of MAALs were recorded. For 
      chromosome characterization, Snow's carmine, fluorescence in situ hybridization 
      (FISH) and genomic in situ hybridization (GISH) were used. KEY RESULTS: The final 
      MAAL was developed. Morphological traits that differentiated the MAALs comprised 
      cotyledon number, leaf morphology, flower colour and seed colour. Seed colour was 
      controlled by major genes on two B. oleracea chromosomes and minor genes on five 
      other chromosomes of this species. Homoeologous pairing was largely between 
      chromosomes with similar centromeric positions. FISH, GISH and a parallel 
      microsatellite marker analysis defined the chromosomes in terms of their linkage 
      groups. Conclusions A complete set of MAALs is now available for genetic, 
      genomic, evolutionary and breeding perspectives. Defining chromosomes that carry 
      specific genes, physical localization of DNA markers and access to established 
      genetic linkage maps contribute to the integration of these approaches, 
      manifested in the confirmed correspondence of linkage groups with specific 
      chromosomes. Applications include marker-assisted selection and breeding for 
      yellow seeds.
FAU - Heneen, Waheeb K
AU  - Heneen WK
AD  - Department of Plant Breeding and Biotechnology, Swedish University of 
      Agricultural Sciences, Alnarp, Sweden. Waheeb.Heneen@slu.se
FAU - Geleta, Mulatu
AU  - Geleta M
FAU - Brismar, Kerstin
AU  - Brismar K
FAU - Xiong, Zhiyong
AU  - Xiong Z
FAU - Pires, J Chris
AU  - Pires JC
FAU - Hasterok, Robert
AU  - Hasterok R
FAU - Stoute, Andrew I
AU  - Stoute AI
FAU - Scott, Roderick J
AU  - Scott RJ
FAU - King, Graham J
AU  - King GJ
FAU - Kurup, Smita
AU  - Kurup S
LA  - eng
GR  - BB/F009712/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/F009721/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Ann Bot
JT  - Annals of botany
JID - 0372347
SB  - IM
MH  - Aneuploidy
MH  - Brassica rapa/embryology/*genetics
MH  - *Chromosomes, Plant
MH  - *Color
MH  - *Genetic Linkage
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
PMC - PMC3359914
EDAT- 2012/05/26 06:00
MHDA- 2012/09/18 06:00
PMCR- 2013/06/01
CRDT- 2012/05/26 06:00
PHST- 2012/05/26 06:00 [entrez]
PHST- 2012/05/26 06:00 [pubmed]
PHST- 2012/09/18 06:00 [medline]
PHST- 2013/06/01 00:00 [pmc-release]
AID - mcs052 [pii]
AID - 10.1093/aob/mcs052 [doi]
PST - ppublish
SO  - Ann Bot. 2012 Jun;109(7):1227-42. doi: 10.1093/aob/mcs052.