PMID- 22630829
OWN - NLM
STAT- MEDLINE
DCOM- 20140602
LR  - 20211021
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 43
IP  - 1
DP  - 2012 May 25
TI  - Interaction of the European genotype porcine reproductive and respiratory 
      syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar 
      macrophage phagocytosis.
PG  - 47
LID - 10.1186/1297-9716-43-47 [doi]
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) is an arterivirus 
      that shows a restricted in vivo tropism for subsets of porcine macrophages, with 
      alveolar macrophages being major target cells. The virus is associated with 
      respiratory problems in pigs of all ages and is commonly isolated on farms with 
      porcine respiratory disease complex (PRDC). Due to virus-induced macrophage death 
      early in infection, PRRSV hampers the innate defence against pathogens in the 
      lungs. In addition, the virus might also directly affect the antimicrobial 
      functions of macrophages. This study examined whether interaction of European 
      genotype PRRSV with primary alveolar macrophages (PAM) affects their phagocytic 
      capacity. Inoculation of macrophages with both subtype I PRRSV (LV) and subtype 
      III PRRSV (Lena) showed that the virus inhibits PAM phagocytosis. Similar results 
      were obtained using inactivated PRRSV (LV), showing that initial interaction of 
      the virion with the cell is sufficient to reduce phagocytosis, and that no 
      productive infection is required. When macrophages were incubated with 
      sialoadhesin- (Sn) or CD163-specific antibodies, two entry mediators of the 
      virus, only Sn-specific antibodies downregulated the phagocytic capacity of PAM, 
      indicating that interaction with Sn, but not CD163, mediates the inhibitory 
      effect of PRRSV on phagocytosis. In conclusion, this study shows that European 
      genotype PRRSV inhibits PAM phagocytosis in vitro, through the interaction with 
      its internalization receptor Sn. If similar events occur in vivo, this 
      interaction may be important in the development of PRDC, as often seen in the 
      field.
FAU - De Baere, Miet I
AU  - De Baere MI
AD  - Laboratory of Virology, Department of Virology, Parasitology and Immunology, 
      Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, 
      Merelbeke, Belgium. Hans.Nauwynck@Ugent.be.
FAU - Van Gorp, Hanne
AU  - Van Gorp H
FAU - Delputte, Peter L
AU  - Delputte PL
FAU - Nauwynck, Hans J
AU  - Nauwynck HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120525
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
SB  - IM
MH  - Animals
MH  - Macrophages, Alveolar/*immunology
MH  - *Phagocytosis
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Sialic Acid Binding Ig-like Lectin 1/*genetics/metabolism
MH  - Swine
PMC - PMC3403922
EDAT- 2012/05/29 06:00
MHDA- 2014/06/03 06:00
PMCR- 2012/01/01
CRDT- 2012/05/29 06:00
PHST- 2012/01/30 00:00 [received]
PHST- 2012/05/25 00:00 [accepted]
PHST- 2012/05/29 06:00 [entrez]
PHST- 2012/05/29 06:00 [pubmed]
PHST- 2014/06/03 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - 1297-9716-43-47 [pii]
AID - 10.1186/1297-9716-43-47 [doi]
PST - epublish
SO  - Vet Res. 2012 May 25;43(1):47. doi: 10.1186/1297-9716-43-47.