PMID- 22633482
OWN - NLM
STAT- MEDLINE
DCOM- 20131209
LR  - 20220316
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 160
IP  - 1-2
DP  - 2012 Nov 9
TI  - Co-infection of porcine dendritic cells with porcine circovirus type 2a (PCV2a) 
      and genotype II porcine reproductive and respiratory syndrome virus (PRRSV) 
      induces CD4(+)CD25(+)FoxP3(+) T cells in vitro.
PG  - 233-9
LID - S0378-1135(12)00289-1 [pii]
LID - 10.1016/j.vetmic.2012.04.040 [doi]
AB  - Porcine circovirus associated disease (PCVAD) is currently one of the most 
      economically important diseases in the global swine industry. Porcine circovirus 
      type 2 (PCV2) is the primary causative agent, however co-infection with other 
      swine pathogens such as porcine reproductive and respiratory syndrome virus 
      (PRRSV) is often required to induce the full spectrum of clinical PCVAD. While 
      the specific mechanisms of viral co-infection that lead to clinical disease are 
      not fully understood, immune modulation by the co-infecting viruses likely plays 
      a critical role. We evaluated the ability of dendritic cells (DC) infected with 
      PRRSV, PCV2, or both to induce regulatory T cells (T(regs)) in vitro. DCs 
      infected with PCV2 significantly increased CD4(+)CD25(+)FoxP3(+) T(regs) (p<0.05) 
      and DCs co-infected with PRRSV and PCV2 induced significantly higher numbers of 
      T(regs) than with PCV2 alone (p<0.05). Cytokine analysis indicated that the 
      induction of T(regs) by co-infected DCs may be dependent on TGF-beta and not IL-10. 
      Our data support the immunomodulatory role of PCV2/PRRSV co-infection in the 
      pathogenesis of PCVAD, specifically via T(reg)-mediated immunosuppression.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Cecere, T E
AU  - Cecere TE
AD  - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional 
      College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24061, USA. 
      tcecere@vt.edu
FAU - Meng, X J
AU  - Meng XJ
FAU - Pelzer, K
AU  - Pelzer K
FAU - Todd, S M
AU  - Todd SM
FAU - Beach, N M
AU  - Beach NM
FAU - Ni, Y Y
AU  - Ni YY
FAU - Leroith, T
AU  - Leroith T
LA  - eng
GR  - T32 RR021819/RR/NCRR NIH HHS/United States
GR  - 5T32RR021819/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120508
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Cytokines)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Circovirus/*immunology
MH  - Coinfection
MH  - Cytokines/immunology
MH  - Dendritic Cells/immunology/pathology/*virology
MH  - Genotype
MH  - Interleukin-10/genetics
MH  - Porcine Postweaning Multisystemic Wasting Syndrome/*immunology/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - T-Lymphocytes, Regulatory/immunology/pathology
MH  - Transforming Growth Factor beta/genetics
PMC - PMC3443269
MID - NIHMS380922
EDAT- 2012/05/29 06:00
MHDA- 2013/12/16 06:00
PMCR- 2012/05/08
CRDT- 2012/05/29 06:00
PHST- 2011/10/12 00:00 [received]
PHST- 2012/04/23 00:00 [revised]
PHST- 2012/04/30 00:00 [accepted]
PHST- 2012/05/29 06:00 [entrez]
PHST- 2012/05/29 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2012/05/08 00:00 [pmc-release]
AID - S0378-1135(12)00289-1 [pii]
AID - 10.1016/j.vetmic.2012.04.040 [doi]
PST - ppublish
SO  - Vet Microbiol. 2012 Nov 9;160(1-2):233-9. doi: 10.1016/j.vetmic.2012.04.040. Epub 
      2012 May 8.