PMID- 22633978
OWN - NLM
STAT- MEDLINE
DCOM- 20130306
LR  - 20211021
IS  - 1872-6623 (Electronic)
IS  - 0304-3959 (Print)
IS  - 0304-3959 (Linking)
VI  - 153
IP  - 10
DP  - 2012 Oct
TI  - Plasticity of cytochrome P450 isozyme expression in rat trigeminal ganglia 
      neurons during inflammation.
PG  - 2031-2039
LID - 10.1016/j.pain.2012.04.027 [doi]
AB  - Recently, specific oxidized linoleic acid metabolites (OLAMs) have been 
      identified as transient receptor potential vanilloid 1 (TRPV1) channel agonists 
      that contribute to inflammatory and heat hyperalgesia mechanisms, yet the 
      specific mechanism responsible for OLAM synthesis in sensory neurons is unknown. 
      Here, we use molecular, anatomical, calcium imaging, and perforated patch 
      electrophysiology methods to demonstrate the specific involvement of cytochrome 
      P450 enzymes (CYPs) in the oxidation of linoleic acid leading to neuronal 
      activation and show that this is enhanced under inflammatory conditions. 
      Additional studies evaluated CYP expressions in the native rat trigeminal ganglia 
      (TG) tissue and cultures as well as changes in their expression pattern following 
      the induction of peripheral inflammation. Fourteen of 20 candidate transcripts 
      were detected in native TG, and 7 of these displayed altered expression under 
      cultured conditions. Moreover, complete Freund's adjuvant-induced inflammation of 
      vibrissal pad selectively increased expression of CYP3A23/3A1 and CYP2J4 
      transcripts in TG. In situ hybridization studies demonstrated broad expression 
      pattern of CYP3A23/3A1 and CYP2J4 within TG neurons. Anatomical studies 
      characterized the expression of CYP3A1 and the CYP2J families within TG sensory 
      neurons, including those with TRPV1, with about half of all TRPV1-positive 
      neurons showing more prominent CYP3A1 and CYP2J expression. Together, these 
      findings show that CYP enzymes play a primary role in mediating linoleic 
      acid-evoked activation of sensory neurons and furthermore, implicate the 
      involvement of specific CYPs as contributing to the formation of OLAMs that act 
      as TRPV1 agonists within this subpopulation of nociceptors.
CI  - Copyright (c) 2012 International Association for the Study of Pain. Published by 
      Elsevier B.V. All rights reserved.
FAU - Ruparel, Shivani
AU  - Ruparel S
AD  - Department of Endodontics, University of Texas Health Science Center at San 
      Antonio, San Antonio, TX, USA Division of Intramural Research, National Institute 
      of Environmental Health Science (NIEHS), National Institutes of Health, Research 
      Triangle Park, NC, USA Department of Biochemistry, University of Texas Health 
      Science Center at San Antonio, San Antonio, TX, USA.
FAU - Henry, Michael A
AU  - Henry MA
FAU - Akopian, Armen
AU  - Akopian A
FAU - Patil, Mayur
AU  - Patil M
FAU - Zeldin, Darryl C
AU  - Zeldin DC
FAU - Roman, Linda
AU  - Roman L
FAU - Hargreaves, Kenneth M
AU  - Hargreaves KM
LA  - eng
GR  - R01DA019585/DA/NIDA NIH HHS/United States
GR  - Z01 ES025034/Intramural NIH HHS/United States
GR  - UL1 TR000149/TR/NCATS NIH HHS/United States
GR  - UL1 TR001120/TR/NCATS NIH HHS/United States
GR  - R01 DA019585/DA/NIDA NIH HHS/United States
GR  - R01 NS072890/NS/NINDS NIH HHS/United States
GR  - R01NS72890/NS/NINDS NIH HHS/United States
GR  - UL1 RR025767/RR/NCRR NIH HHS/United States
GR  - U54RR02438/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
DEP - 20120526
PL  - United States
TA  - Pain
JT  - Pain
JID - 7508686
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - 9KJL21T0QJ (Linoleic Acid)
SB  - IM
CIN - Pain. 2012 Oct;153(10):1987-8. PMID: 22681878
MH  - Animals
MH  - Cytochrome P-450 Enzyme System/*metabolism
MH  - Gene Expression Regulation, Enzymologic
MH  - Hyperalgesia/complications/*metabolism
MH  - Inflammation/etiology/*metabolism
MH  - Linoleic Acid/*metabolism
MH  - Male
MH  - *Neuronal Plasticity
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sensory Receptor Cells/*metabolism
MH  - Trigeminal Ganglion/*enzymology
PMC - PMC3430817
MID - NIHMS374509
COIS- The authors have declared that no other conflict of interest exists.
EDAT- 2012/05/29 06:00
MHDA- 2013/03/07 06:00
PMCR- 2013/10/01
CRDT- 2012/05/29 06:00
PHST- 2012/02/09 00:00 [received]
PHST- 2012/04/25 00:00 [revised]
PHST- 2012/04/26 00:00 [accepted]
PHST- 2012/05/29 06:00 [entrez]
PHST- 2012/05/29 06:00 [pubmed]
PHST- 2013/03/07 06:00 [medline]
PHST- 2013/10/01 00:00 [pmc-release]
AID - 00006396-201210000-00010 [pii]
AID - 10.1016/j.pain.2012.04.027 [doi]
PST - ppublish
SO  - Pain. 2012 Oct;153(10):2031-2039. doi: 10.1016/j.pain.2012.04.027. Epub 2012 May 
      26.