PMID- 22634598
OWN - NLM
STAT- MEDLINE
DCOM- 20121226
LR  - 20120827
IS  - 1567-7257 (Electronic)
IS  - 1567-1348 (Linking)
VI  - 12
IP  - 7
DP  - 2012 Oct
TI  - Isolation and characterization of a mannose-binding insecticidal lectin gene from 
      Allium sativum (garlic) and its putative role in insect resistance using 
      bioinformatics tools.
PG  - 1508-12
LID - 10.1016/j.meegid.2012.05.008 [doi]
AB  - Monocot mannose-binding lectins (MMBLs) or agglutinins are an extended 
      superfamily of structurally and evolutionarily related proteins. They play 
      important roles in plant defenses. Here we describe the synthesis of full-length 
      cDNA of monocot mannose-binding insecticidal agglutinin isolated from Allium 
      sativum, a traditional herb known to be of great applications in Africa, using 
      reverse transcription polymerase chain reaction (RT-PCR) with specific primers 
      designed based on the insecticidal sequence (NCBI primary accession no. 
      AY866499.1). Sequence analysis revealed a 327bp open reading frame (ORF) encoding 
      a putative 108-aa agglutinin precursor with a C-terminal domain. Multiple 
      alignments of BLEC1 amino acids with those of eight other MMBLs revealed three 
      highly conserved domains among them, indicating BLEC1 belongs to a member of the 
      MMBL superfamily. Tertiary structure analysis showed that BLEC1 had three 
      potential equal mannose-binding sites. Phylogenetic analysis indicated that 20 
      MMBLs including BLEC1 belonged to an extended superfamily. Gene ontology analyses 
      indicate one biological process with GO ID: 0006952 representing defense 
      response, with two secondary IDs GO: 0002217 GO: 0042829. The child terms has 
      both negative and positive regulation some of which include GO: 0002242 defense 
      response to parasitic plant and GO: 0002213 defense response to insect. The 
      cloning and characterization of BLEC1 will enable us to study its potential use 
      in plant genetic engineering in the development of insect resistance plant.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Afolabi-Balogun, N B
AU  - Afolabi-Balogun NB
AD  - Centre for Biotechnology Research and Training, Ahmadu Bello University, Zaria, 
      Nigeria. nbafolabi-balogun@abu.edu.ng
FAU - Inuwa, H M
AU  - Inuwa HM
FAU - Ishiyaku, M F
AU  - Ishiyaku MF
FAU - Bakare-Odunoola, M T
AU  - Bakare-Odunoola MT
FAU - Nok, A J
AU  - Nok AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120524
PL  - Netherlands
TA  - Infect Genet Evol
JT  - Infection, genetics and evolution : journal of molecular epidemiology and 
      evolutionary genetics in infectious diseases
JID - 101084138
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Plant Lectins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Computational Biology
MH  - Disease Resistance/*genetics
MH  - Garlic/*genetics/immunology
MH  - Mannose-Binding Lectins/chemistry/*genetics/isolation & purification
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Plant Diseases/immunology
MH  - Plant Lectins/chemistry/*genetics/isolation & purification
MH  - Protein Structure, Tertiary
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
EDAT- 2012/05/29 06:00
MHDA- 2012/12/27 06:00
CRDT- 2012/05/29 06:00
PHST- 2011/06/16 00:00 [received]
PHST- 2012/05/07 00:00 [revised]
PHST- 2012/05/13 00:00 [accepted]
PHST- 2012/05/29 06:00 [entrez]
PHST- 2012/05/29 06:00 [pubmed]
PHST- 2012/12/27 06:00 [medline]
AID - S1567-1348(12)00188-8 [pii]
AID - 10.1016/j.meegid.2012.05.008 [doi]
PST - ppublish
SO  - Infect Genet Evol. 2012 Oct;12(7):1508-12. doi: 10.1016/j.meegid.2012.05.008. 
      Epub 2012 May 24.