PMID- 22639808
OWN - NLM
STAT- MEDLINE
DCOM- 20120823
LR  - 20131121
IS  - 0289-0003 (Print)
IS  - 0289-0003 (Linking)
VI  - 29
IP  - 6
DP  - 2012 Jun
TI  - Transforming growth factor-alpha mRNA expression and its possible roles in mouse 
      endometrial stromal cells.
PG  - 377-83
LID - 10.2108/zsj.29.377 [doi]
AB  - Transforming growth factor-alpha (TGFalpha) is thought to be involved in the regulation 
      of endometrial cells. We investigated Tgfa mRNA expression, and the effects of 
      TGFalpha on DNA-synthesis and gene expression of insulin-like growth factor 1 (IGF1), 
      IGF binding protein-3 (IGFBP3) and IGF1 receptor in the mouse endometrial cells, 
      because IGF1 is involved in estrogen-induced growth of endometrial cells. We also 
      investigated the role of TGFalpha on matrix metalloproteinase (MMP) expression, as 
      MMPs are involved both in tissue remodeling during cell proliferation and in 
      enhancement of IGF1 signaling through the degradation of IGFBP3. Tgfa mRNA 
      expression was detected in endometrial luminal and glandular epithelial cells, 
      and stromal cells. Tgfa mRNA signals did not appear to change in endometrial 
      luminal epithelial cells, but signals in glandular epithelial cells were higher 
      at diestrus 1, 2 and proestrus, and the number of stromal cells showing strong 
      signals appeared to increase at diestrus 1 and 2. Endometrial epithelial and 
      stromal cells were treated with estradiol-17beta (E2) or progesterone (P4). E2 or P4 
      stimulated Tgfa mRNA expression in stromal cells. TGFalpha stimulated DNA synthesis 
      in endometrial epithelial and stromal cells, while E2 and P4 stimulated DNA 
      synthesis in stromal cells. In stromal cells, TGFalpha, at as low as 1 ng/ml, 
      decreased Igfbp3 and Mmp9 mRNA levels, while high dose (10 ng/ml) of TGFalpha 
      decreased Igf1 mRNA level and increased Mmp3 mRNA level. These results imply that 
      TGFalpha stimulates proliferation of endometrial stromal cells through multiple 
      mechanisms, including its regulation of Igfbp3 and Mmp3 transcription.
FAU - Maekawa, Tetsuya
AU  - Maekawa T
AD  - Department of Biology, The Graduate School of Science and Technology, Okayama 
      University, Tsushima, Kita-ku, Okayama 700-8530, Japan.
FAU - Sakuma, Atsuko
AU  - Sakuma A
FAU - Taniuchi, Shusuke
AU  - Taniuchi S
FAU - Ogo, Yuki
AU  - Ogo Y
FAU - Iguchi, Taisen
AU  - Iguchi T
FAU - Takeuchi, Sakae
AU  - Takeuchi S
FAU - Takahashi, Sumio
AU  - Takahashi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Zoolog Sci
JT  - Zoological science
JID - 8702287
RN  - 0 (Transforming Growth Factor alpha)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 63231-63-0 (RNA)
RN  - EC 3.4.24.- (Matrix Metalloproteinases, Secreted)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Cells, Cultured
MH  - Endometrium/*cytology
MH  - Epithelial Cells/drug effects/metabolism
MH  - Estradiol/pharmacology
MH  - Estrous Cycle/physiology
MH  - Female
MH  - Gene Expression Regulation/physiology
MH  - Matrix Metalloproteinases, Secreted/genetics/metabolism
MH  - Mice
MH  - Progesterone/pharmacology
MH  - RNA/genetics/metabolism
MH  - Stromal Cells/drug effects/*metabolism
MH  - Transforming Growth Factor alpha/genetics/*metabolism
EDAT- 2012/05/30 06:00
MHDA- 2012/08/24 06:00
CRDT- 2012/05/30 06:00
PHST- 2012/05/30 06:00 [entrez]
PHST- 2012/05/30 06:00 [pubmed]
PHST- 2012/08/24 06:00 [medline]
AID - 10.2108/zsj.29.377 [doi]
PST - ppublish
SO  - Zoolog Sci. 2012 Jun;29(6):377-83. doi: 10.2108/zsj.29.377.