PMID- 22639915
OWN - NLM
STAT- MEDLINE
DCOM- 20121030
LR  - 20131121
IS  - 1464-410X (Electronic)
IS  - 1464-4096 (Linking)
VI  - 110
IP  - 6 Pt B
DP  - 2012 Sep
TI  - Green tea catechins decrease oxidative stress in surgical menopause-induced 
      overactive bladder in a rat model.
PG  - E236-44
LID - 10.1111/j.1464-410X.2012.11258.x [doi]
AB  - What's known on the subject? and What does the study add? Ovary hormone 
      deficiency and the age-related changes in post-menopausal women are subjected to 
      a number of urological dysfunctions, including overactive bladder syndrome. Green 
      tea is a popular healthy drink worldwide and its extract catechin has strong 
      anti-inflammatory and antioxidant properties. EGCG, the major type of catechin, 
      is an antioxidant polyphenol flavonoid isolated from green tea. EGCG supplement 
      could prevent ovariectomy-induced bladder dysfunction in a dose-related manner 
      through its anti-oxidant, anti-fibrosis and anti-apoptosis effects. OBJECTIVE: To 
      evaluate whether green tea extract, epigallocatechin gallate (EGCG), could 
      prevent ovariectomy-induced overactive bladder (OAB) and to investigate its 
      antioxidant, anti-apoptotic and anti-fibrosis effects. MATERIALS AND METHODS: In 
      all, 48 female Sprague-Dawley rats were divided into four groups. After bilateral 
      ovariectomy, the first group served as the ovariectomy control, the second group 
      received EGCG 1 microM/kg daily i.p. injection after ovariectomy surgery, and the 
      third group received EGCG 10 microM/kg daily i.p. injection. The fourth group was 
      taken as the sham without ovariectomy surgery. The rats were killed after 6 
      months after ovariectomy surgery. Cystometrograms were performed for the measure 
      of bladder overactivity. Terminal deoxynucleotidyl transferase-mediated 
      dUTP-biotin nick-end labelling (TUNEL) assay was used to evaluate apoptotic 
      cells. Western immunoblots were performed to determine the expressions of 
      inflammatory markers, apoptosis-associated proteins and oxidative stress markers. 
      RESULTS: Long-term ovariectomy significantly increased non-voiding contractions 
      and decreased bladder compliance. Treatment with EGCG significantly increased 
      bladder compliance and diminished non-voiding contractions. Ovariectomy 
      significantly increased apoptotic cells and enhanced interstitial fibrosis in 
      bladders. The expression of caspase-3 significantly increased, while that of 
      Bcl-2 notably decreased after ovariectomy. Inflammatory and fibrosis markers, 
      TGF-beta, fibronectin and type I collagen expressions were significantly increased 
      after 6 months of ovariectomy surgery. Treatment with EGCG significantly 
      decreased TGF-beta and type I collagen expressions. Oxidative stress markers, 
      nitrotyrosine and protein carbonylation levels were significantly increased in 
      the ovariectomy group. EGCG could attenuate this oxidative damage in 
      dose-dependent fashion. CONCLUSIONS: Ovariectomy increased oxidative damage, 
      enhanced voiding frequency and decreased bladder compliance. EGCG could restore 
      ovariectomy-induced bladder dysfunction in a dose-dependent fashion through 
      antioxidant, anti-fibrosis and anti-apoptosis effects.
CI  - (c) 2012 THE AUTHORS. BJU INTERNATIONAL (c) 2012 BJU INTERNATIONAL.
FAU - Juan, Yung-Shun
AU  - Juan YS
AD  - Department of Urology, College of Medicine, Kaohsiung Medical University, 
      Kaohsiung, Taiwan.
FAU - Chuang, Shu-Mien
AU  - Chuang SM
FAU - Lee, Yi-Lun
AU  - Lee YL
FAU - Long, Cheng-Yu
AU  - Long CY
FAU - Wu, Tzu-Hui
AU  - Wu TH
FAU - Chang, Wei-Chiao
AU  - Chang WC
FAU - Levin, Robert M
AU  - Levin RM
FAU - Liu, Keh-Min
AU  - Liu KM
FAU - Huang, Chun-Hsiung
AU  - Huang CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120528
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
RN  - 0 (Antioxidants)
RN  - 0 (Plant Extracts)
RN  - 0 (Tea)
RN  - 8R1V1STN48 (Catechin)
RN  - BQM438CTEL (epigallocatechin gallate)
SB  - IM
CIN - Nat Rev Urol. 2012 Jul;9(7):353. PMID: 22710666
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Catechin/*analogs & derivatives/pharmacology
MH  - Disease Models, Animal
MH  - Female
MH  - Menopause/*metabolism
MH  - Ovariectomy
MH  - Oxidative Stress/*drug effects
MH  - Plant Extracts/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Tea
MH  - Urinary Bladder, Overactive/*metabolism
EDAT- 2012/05/30 06:00
MHDA- 2012/10/31 06:00
CRDT- 2012/05/30 06:00
PHST- 2012/05/30 06:00 [entrez]
PHST- 2012/05/30 06:00 [pubmed]
PHST- 2012/10/31 06:00 [medline]
AID - 10.1111/j.1464-410X.2012.11258.x [doi]
PST - ppublish
SO  - BJU Int. 2012 Sep;110(6 Pt B):E236-44. doi: 10.1111/j.1464-410X.2012.11258.x. 
      Epub 2012 May 28.