PMID- 22642151
OWN - NLM
STAT- MEDLINE
DCOM- 20120904
LR  - 20191112
IS  - 2310-6972 (Print)
IS  - 2310-6905 (Linking)
VI  - 58
IP  - 1
DP  - 2012 Jan-Feb
TI  - [Thermodynamic analysis of dimerization inhibitors binding to HIV protease 
      monomers].
PG  - 43-9
AB  - Here, we describe the analysis of kinetic and thermodynamic parameters for 
      binding of peptide and nonpeptide dimerization inhibitors to immobilized HIV 
      protease (HIVp) monomers by using surface plasmon resonance. Molecular 
      interactions were investigated at different inhibitors concentrations (0-80 
      microM) and temperatures (15-35 degrees C). The kinetic, equilibrium and 
      thermodynamic parameters have been determined. It was found that both inhibitors 
      were characterized by similar interaction parameters. The complex formation is 
      entropically driven process for both inhibitors. The entropic term(-TdeltaS) had 
      the value about -20 kcal/mol while the enthalpic term (deltaH) had the positive 
      value about 14 kcal/mol and counteracted the complex formation.
FAU - Ershov, P V
AU  - Ershov PV
FAU - Gnedenko, O V
AU  - Gnedenko OV
FAU - Mol'nar, A A
AU  - Mol'nar AA
FAU - Lisitsa, A V
AU  - Lisitsa AV
FAU - Ivanov, A S
AU  - Ivanov AS
FAU - Archakov, A I
AU  - Archakov AI
LA  - rus
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Biomed Khim
JT  - Biomeditsinskaia khimiia
JID - 101196966
RN  - 0 (HIV Protease Inhibitors)
RN  - EC 3.4.23.- (HIV Protease)
SB  - IM
MH  - Dimerization
MH  - HIV Protease/*chemistry
MH  - HIV Protease Inhibitors/*chemistry
MH  - Humans
MH  - Kinetics
MH  - Protein Binding
MH  - Protein Multimerization/*drug effects
MH  - Surface Plasmon Resonance/*methods
MH  - Thermodynamics
EDAT- 2012/05/31 06:00
MHDA- 2012/09/05 06:00
CRDT- 2012/05/31 06:00
PHST- 2012/05/31 06:00 [entrez]
PHST- 2012/05/31 06:00 [pubmed]
PHST- 2012/09/05 06:00 [medline]
AID - 10.18097/pbmc20125801043 [doi]
PST - ppublish
SO  - Biomed Khim. 2012 Jan-Feb;58(1):43-9. doi: 10.18097/pbmc20125801043.