PMID- 22642927
OWN - NLM
STAT- MEDLINE
DCOM- 20130102
LR  - 20120905
IS  - 1463-5224 (Electronic)
IS  - 1463-5216 (Linking)
VI  - 15 Suppl 2
DP  - 2012 Sep
TI  - Characterization of structure and function of the mouse retina using pattern 
      electroretinography, pupil light reflex, and optical coherence tomography.
PG  - 94-104
LID - 10.1111/j.1463-5224.2012.01034.x [doi]
AB  - OBJECTIVE: To perform in vivo analysis of retinal functional and structural 
      parameters in healthy mouse eyes. ANIMAL STUDIED: Adult C57BL/6 male mice (n = 
      37). PROCEDURES: Retinal function was evaluated using pattern electroretinography 
      (pERG) and the chromatic pupil light reflex (cPLR). Structural properties of the 
      retina and nerve fiber layer (NFL) were evaluated using spectral-domain optical 
      coherence tomography (SD-OCT). RESULTS: The average pERG amplitudes were found to 
      be 11.2 +/- 0.7 muV (P50-N95, mean +/- SEM), with an implicit time for P50-N95 
      interval of 90.4 +/- 5.4 ms. Total retinal thickness was 229.5 +/- 1.7 mum (mean +/- 
      SEM) in the area centralis region. The thickness of the retinal nerve fiber layer 
      (mean +/- SEM) using a circular peripapillary retinal scan centered on the optic 
      nerve was 46.7 +/- 0.9 mum (temporal), 46.1 +/- 0.9 mum (superior), 45.8 +/- 0.9 mum 
      (nasal), and 48.4 +/- 1 mum (inferior). The baseline pupil diameter was 2.1 +/- 0.05 
      mm in darkness, and 1.1 +/- 0.05 and 0.56 +/- 0.03 mm after stimulation with red (630 
      nm, luminance 200 kcd/m(2)) or blue (480 nm, luminance 200 kcd/m(2)) light 
      illumination, respectively. CONCLUSIONS: Pattern electroretinography, cPLR and 
      SD-OCT analysis are reproducible techniques, which can provide important 
      information about retinal and optic nerve function and structure in mice.
CI  - (c) 2012 American College of Veterinary Ophthalmologists.
FAU - Mohan, Kabhilan
AU  - Mohan K
AD  - Iowa City Department of Veterans Affairs Center for Prevention and Treatment of 
      Vision Loss, Iowa City, IA 52246-2209, USA.
FAU - Harper, Matthew M
AU  - Harper MM
FAU - Kecova, Helga
AU  - Kecova H
FAU - Ye, Eun-Ah
AU  - Ye EA
FAU - Lazic, Tatjana
AU  - Lazic T
FAU - Sakaguchi, Donald S
AU  - Sakaguchi DS
FAU - Kardon, Randy H
AU  - Kardon RH
FAU - Grozdanic, Sinisa D
AU  - Grozdanic SD
CN  - CR: BioMed Vision Technologies, Neuroptics Inc
LA  - eng
GR  - 1R01EY019294/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120529
PL  - England
TA  - Vet Ophthalmol
JT  - Veterinary ophthalmology
JID - 100887377
SB  - IM
MH  - Animals
MH  - Electroretinography
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Optic Nerve/physiology
MH  - Reflex, Pupillary/*physiology
MH  - Retina/*anatomy & histology/*physiology
MH  - Tomography, Optical Coherence/*methods
EDAT- 2012/05/31 06:00
MHDA- 2013/01/03 06:00
CRDT- 2012/05/31 06:00
PHST- 2012/05/31 06:00 [entrez]
PHST- 2012/05/31 06:00 [pubmed]
PHST- 2013/01/03 06:00 [medline]
AID - 10.1111/j.1463-5224.2012.01034.x [doi]
PST - ppublish
SO  - Vet Ophthalmol. 2012 Sep;15 Suppl 2:94-104. doi: 
      10.1111/j.1463-5224.2012.01034.x. Epub 2012 May 29.