PMID- 22643330
OWN - NLM
STAT- MEDLINE
DCOM- 20130325
LR  - 20130307
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 93
IP  - 12
DP  - 2012 Jun 27
TI  - Reconstitution of 6-sulfo LacNAc dendritic cells after allogeneic stem-cell 
      transplantation.
PG  - 1270-5
LID - 10.1097/TP.0b013e31824fd8b4 [doi]
AB  - BACKGROUND: Infections and acute graft-versus-host disease (GvHD) represent major 
      complications of allogeneic stem-cell transplantation (SCT). Dendritic cells 
      (DCs) display an extraordinary capacity to induce innate and adaptive immune 
      responses. Therefore, they play a crucial role in the elimination of pathogens 
      and in the pathogenesis of acute GvHD. 6-Sulfo LacNAc DCs (slanDCs) are a major 
      subpopulation of human blood DCs with a high proinflammatory capacity. We 
      investigated for the first time the reconstitution of slanDCs in the blood of 
      patients after SCT and the modulation of their frequency by bacterial infection, 
      cytomegalovirus (CMV) reactivation, and acute GvHD. METHODS: The frequency of 
      slanDCs, CD1c myeloid DCs (mDCs), and plasmacytoid DCs (pDCs) in the peripheral 
      blood was quantified by flow cytometry in 80 patients after SCT. To assess 
      individual DC subsets, we used pregating of the HLADRLin subset and antibodies 
      against slanDCs, blood DC antigen 1 (CD1c mDCs), and blood DC antigen 2 (pDCs). 
      RESULTS: SlanDCs showed the slowest reconstitution in the first month after SCT 
      compared with CD1c mDCs and pDCs. Interestingly, in the second and third months 
      after SCT, their percentage steadily increased, and slanDCs were the most 
      abundant DC subset. In addition, we observed a markedly reduced frequency of 
      slanDCs in the blood of patients with bacterial infection, CMV reactivation, or 
      severe acute GvHD. Furthermore, slanDCs showed the most prominent reduction after 
      steroid treatment of acute GvHD. CONCLUSIONS: These results indicate that 
      SCT-associated complications such as bacterial infection, CMV reactivation, and 
      acute GvHD can significantly modulate the frequency of slanDCs.
FAU - Mager, Konrad
AU  - Mager K
AD  - Department of Medicine I, University Hospital Carl Gustav Carus, Dresden, 
      Germany.
FAU - Wehner, Rebekka
AU  - Wehner R
FAU - Bahr, Felix
AU  - Bahr F
FAU - Oelschlagel, Uta
AU  - Oelschlagel U
FAU - Platzbecker, Uwe
AU  - Platzbecker U
FAU - Wermke, Martin
AU  - Wermke M
FAU - Shayegi, Nona
AU  - Shayegi N
FAU - Middeke, Jan Moritz
AU  - Middeke JM
FAU - Radke, Jorgen
AU  - Radke J
FAU - Rollig, Christoph
AU  - Rollig C
FAU - Schetelig, Johannes
AU  - Schetelig J
FAU - Thiede, Christian
AU  - Thiede C
FAU - Ehninger, Gerhard
AU  - Ehninger G
FAU - Schmitz, Marc
AU  - Schmitz M
FAU - Bornhauser, Martin
AU  - Bornhauser M
FAU - Tuve, Sebastian
AU  - Tuve S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (6-sulfo-LacNac)
RN  - 0 (Amino Sugars)
RN  - 0 (Autoantibodies)
RN  - 0 (Steroids)
SB  - IM
MH  - Acute Disease
MH  - Adaptive Immunity/immunology
MH  - Adult
MH  - Aged
MH  - Amino Sugars/*metabolism
MH  - Autoantibodies/blood/immunology
MH  - Bacterial Infections/immunology
MH  - Cohort Studies
MH  - Cytomegalovirus Infections/immunology
MH  - Dendritic Cells/cytology/*immunology/*metabolism
MH  - Female
MH  - Graft vs Host Disease/drug therapy/*immunology
MH  - Humans
MH  - Immunity, Innate/immunology
MH  - Male
MH  - Middle Aged
MH  - Stem Cell Transplantation/*adverse effects
MH  - Steroids/therapeutic use
MH  - Transplantation, Homologous
MH  - Young Adult
EDAT- 2012/05/31 06:00
MHDA- 2013/03/26 06:00
CRDT- 2012/05/31 06:00
PHST- 2012/05/31 06:00 [entrez]
PHST- 2012/05/31 06:00 [pubmed]
PHST- 2013/03/26 06:00 [medline]
AID - 10.1097/TP.0b013e31824fd8b4 [doi]
PST - ppublish
SO  - Transplantation. 2012 Jun 27;93(12):1270-5. doi: 10.1097/TP.0b013e31824fd8b4.