PMID- 22643406
OWN - NLM
STAT- MEDLINE
DCOM- 20120727
LR  - 20120530
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 65
IP  - 12
DP  - 2012
TI  - Characterization of microbial community structures and their activities in single 
      anaerobic granules by beta imaging, microsensors and fluorescence in situ 
      hybridization.
PG  - 2125-31
LID - 10.2166/wst.2012.117 [doi]
AB  - The spatial distribution of microorganisms and their in situ activities in 
      anaerobic granules were investigated by fluorescence in situ hybridization 
      (FISH), beta imaging and microsensors. FISH results revealed a layered structure 
      of microorganisms in the granule, where Chloroflexi was present in the outermost 
      layer, Smithella spp. and Syntrophobacter spp. were found in a depth of ca. 100 
      mum, and Archaea was restricted to the inner layer (below ca. 300 mum from the 
      surface). Substrate uptake patterns elucidated by beta imaging demonstrated that 
      glucose uptake was highest at 50 mum depth, whereas propionate uptake had a peak 
      at 200 mum depth. In addition, microsensor measurements revealed that acid was 
      produced mainly at 100 mum depth and H(2) production was detected at a depth from 
      100 to 200 mum. H(2) consumption and corresponding CH(4) production were found 
      below 200 mum from the surface. Direct comparison of these results implied 
      sequential degradation of complex organic compounds in anaerobic granules; 
      Chloroflexi contributed to fermentation of organic compounds and acid production 
      in the outermost layer, volatile fatty acids were oxidized and H(2) was produced 
      mainly by Smithella spp. and Syntrophobacter spp. at a depth from 100 to 200 mum, 
      and Archaea produced CH(4) below ca. 300 mum from the surface.
FAU - Satoh, H
AU  - Satoh H
AD  - Hokkaido University, Sapporo, Japan. qsatoh@eng.hokudai.ac.jp
FAU - Tsushima, I
AU  - Tsushima I
FAU - Miura, Y
AU  - Miura Y
FAU - Ito, T
AU  - Ito T
FAU - Okabe, S
AU  - Okabe S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
SB  - IM
MH  - Anaerobiosis
MH  - Chloroflexi/genetics/*metabolism
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Proteobacteria/genetics/*metabolism
EDAT- 2012/05/31 06:00
MHDA- 2012/07/28 06:00
CRDT- 2012/05/31 06:00
PHST- 2012/05/31 06:00 [entrez]
PHST- 2012/05/31 06:00 [pubmed]
PHST- 2012/07/28 06:00 [medline]
AID - 10.2166/wst.2012.117 [doi]
PST - ppublish
SO  - Water Sci Technol. 2012;65(12):2125-31. doi: 10.2166/wst.2012.117.