PMID- 22643829
OWN - NLM
STAT- MEDLINE
DCOM- 20121213
LR  - 20211021
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 61
IP  - 8
DP  - 2012 Aug
TI  - Use of blood urea nitrogen, creatinine, interleukin-6, granulocyte-macrophage 
      colony stimulating factor in combination to predict the severity and outcome of 
      abdominal sepsis in rats.
PG  - 889-97
LID - 10.1007/s00011-012-0481-3 [doi]
AB  - OBJECTIVES: Accurate and timely diagnosis and prognosis of sepsis remain 
      challenging. A combination of markers, as opposed to single ones, may improve the 
      prognosis, and therefore survival. This study compared the effectiveness of 
      routinely used biomarkers of sepsis alone and in combination for the prediction 
      of outcome in rats with abdominal sepsis. METHODS: Rats were subjected to sepsis 
      induced by cecal ligation and puncture (CLP). Seventeen biomarkers were detected 
      12 h after the CLP. Correlation between the biomarkers and outcome of rats was 
      analyzed; the correlated biomarkers were analyzed by logistic regression analysis 
      and the area under the receiver operating characteristic (ROC) curve was computed 
      to compare their performance in the prognosis of sepsis. RESULTS: A total of 49 
      rats were eligible for analysis. Body temperature (T), blood urea nitrogen (BUN), 
      creatinine (Cr), alanine aminotransferase (ALT), creatine kinase (CK), 
      interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) levels after 
      the CLP were negatively correlated with the survival outcome, while platelet 
      count (PLT), high-mobility group box protein 1 (HMGB1) and granulocyte-macrophage 
      colony stimulating factor (GM-CCF) were positively correlated with the survival 
      outcome (P < 0.05). Levels of BUN, Cr, IL-6, and GM-CSF after the CLP were 
      independent predictors of outcome according to conditional logistic regression. 
      The sensitivity and specificity of the four selected biomarkers in combination 
      for predicting sepsis outcome were better than single ones (P < 0.05). 
      CONCLUSION: A combination of different biomarkers improves the diagnostic 
      accuracy and is more effective in the prognosis of sepsis in rats. Use of BUN, 
      Cr, IL-6, GM-CSF in combination to predict the severity and outcome in rats with 
      abdominal sepsis exhibited acceptable diagnostic characteristics.
FAU - Gao, Min
AU  - Gao M
AD  - Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, 
      Central South University, Changsha, Hunan, People's Republic of China.
FAU - Zhang, Lingli
AU  - Zhang L
FAU - Liu, Ying
AU  - Liu Y
FAU - Yang, Mingshi
AU  - Yang M
FAU - Wang, Nian
AU  - Wang N
FAU - Wang, Kangkai
AU  - Wang K
FAU - Ou, Danmin
AU  - Ou D
FAU - Liu, Meidong
AU  - Liu M
FAU - Chen, Guangwen
AU  - Chen G
FAU - Liu, Ke
AU  - Liu K
FAU - Xiao, Xianzhong
AU  - Xiao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120529
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Biomarkers)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (HMGB1 Protein)
RN  - 0 (Hbp1 protein, rat)
RN  - 0 (Interleukin-6)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - AYI8EX34EU (Creatinine)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - EC 2.7.3.2 (Creatine Kinase)
SB  - IM
MH  - Abdomen
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Biomarkers
MH  - *Blood Urea Nitrogen
MH  - Chemokine CCL2/blood
MH  - Creatine Kinase/blood
MH  - Creatinine/*blood
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*blood
MH  - HMGB1 Protein/blood
MH  - Interleukin-6/*blood
MH  - Male
MH  - Platelet Count
MH  - Prognosis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sepsis/*blood
EDAT- 2012/05/31 06:00
MHDA- 2012/12/14 06:00
CRDT- 2012/05/31 06:00
PHST- 2011/10/14 00:00 [received]
PHST- 2012/04/15 00:00 [accepted]
PHST- 2012/04/10 00:00 [revised]
PHST- 2012/05/31 06:00 [entrez]
PHST- 2012/05/31 06:00 [pubmed]
PHST- 2012/12/14 06:00 [medline]
AID - 10.1007/s00011-012-0481-3 [doi]
PST - ppublish
SO  - Inflamm Res. 2012 Aug;61(8):889-97. doi: 10.1007/s00011-012-0481-3. Epub 2012 May 
      29.