PMID- 22644639
OWN - NLM
STAT- MEDLINE
DCOM- 20121106
LR  - 20240109
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 167
IP  - 7
DP  - 2012 Aug
TI  - Bioactive compounds extracted from Ecklonia cava by using enzymatic hydrolysis 
      protects high glucose-induced damage in INS-1 pancreatic beta-cells.
PG  - 1973-85
LID - 10.1007/s12010-012-9695-7 [doi]
AB  - Pancreatic beta-cells are very sensitive to oxidative stress and this might play an 
      important role in beta-cell death in diabetes. In the present study, we investigated 
      whether the brown alga Ecklonia cava has protective effects against high 
      glucose-induced damage in INS-1 pancreatic beta-cells. For that purpose, we prepared 
      an enzymatic hydrolysate from E. cava (EHE) by using the carbohydrase, 
      Celluclast. High-glucose (30 mM) treatment induced glucotoxicity, whereas EHE 
      prevented cells from high glucose-induced damage then restoring cell viability 
      was significantly increased. Furthermore, lipid peroxidation, intracellular 
      reactive oxygen species (ROS) and nitric oxide (NO) were overproduced as the 
      result of the treatment by high glucose; however, these lipid peroxidation, ROS 
      and NO generations were effectively inhibited by addition of EHE in a 
      dose-dependent manner. Moreover, EHE treatment increased activities of 
      antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD), and 
      glutathione peroxidase (GSH-px) in high glucose pretreated INS-1 pancreatic 
      beta-cells. EHE slightly reduced the expression of pro-apoptotic protein Bax induced 
      by high glucose but increased the expression of Bcl-2, an anti-apoptotic protein. 
      These findings indicate that EHE might be used as potential nutraceutical agent 
      which will protect the glucotoxicity caused by hyperglycemia-induced oxidative 
      stress associated with diabetes.
FAU - Lee, Seung-Hong
AU  - Lee SH
AD  - Department of Marine Life Science, Jeju National University, Jeju 690-756, South 
      Korea.
FAU - Park, Mi-Hwa
AU  - Park MH
FAU - Park, Sun-Joo
AU  - Park SJ
FAU - Kim, Jaeil
AU  - Kim J
FAU - Kim, Yong-Tae
AU  - Kim YT
FAU - Oh, Myung-Cheol
AU  - Oh MC
FAU - Jeong, Yoonhwa
AU  - Jeong Y
FAU - Kim, Misook
AU  - Kim M
FAU - Han, Ji-Sook
AU  - Han JS
FAU - Jeon, You-Jin
AU  - Jeon YJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120529
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Antioxidants)
RN  - 0 (Biological Products)
RN  - 0 (Polyphenols)
RN  - 0 (Polysaccharides)
RN  - 0 (Protective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
RN  - EC 3.2.1.- (carbohydrase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Apoptosis/drug effects
MH  - Biological Products/*isolation & purification/*pharmacology
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Cytoprotection/*drug effects
MH  - Enzyme Assays
MH  - Glucose/*toxicity
MH  - Glycoside Hydrolases/*metabolism
MH  - Hydrolysis/drug effects
MH  - Insulin-Secreting Cells/drug effects/metabolism/*pathology
MH  - Intracellular Space/drug effects/metabolism
MH  - Lipid Peroxidation/drug effects
MH  - Nitric Oxide/biosynthesis
MH  - Phaeophyceae/*chemistry
MH  - Polyphenols/pharmacology
MH  - Polysaccharides/pharmacology
MH  - Protective Agents/pharmacology
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Staining and Labeling
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - bcl-2-Associated X Protein/metabolism
EDAT- 2012/05/31 06:00
MHDA- 2012/11/07 06:00
CRDT- 2012/05/31 06:00
PHST- 2011/09/19 00:00 [received]
PHST- 2012/04/12 00:00 [accepted]
PHST- 2012/05/31 06:00 [entrez]
PHST- 2012/05/31 06:00 [pubmed]
PHST- 2012/11/07 06:00 [medline]
AID - 10.1007/s12010-012-9695-7 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2012 Aug;167(7):1973-85. doi: 10.1007/s12010-012-9695-7. 
      Epub 2012 May 29.