PMID- 22644762
OWN - NLM
STAT- MEDLINE
DCOM- 20130322
LR  - 20211021
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 45
IP  - 2
DP  - 2012 Oct
TI  - Sequence analysis of the NSP2, ORF5, and ORF7 genes of 11 PRRS virus isolates 
      from China.
PG  - 256-64
AB  - During a tracing investigation of blue ear disease in China conducted from 
      January to November 2008, 11 porcine reproductive and respiratory syndrome virus 
      (PRRSV) isolates were collected from eight provinces including Liaoning, Jilin, 
      Hebei, Shandong, Henan, Shanghai, Zhejiang, and Guangxi. The complete gene 
      sequences of the NSP2, ORF5, and ORF7 genes from these 11 PRRSV isolates were 
      amplified, cloned and detected by RT-PCR and then compared with the published 
      sequences of other strains. The results showed that all of the isolates 
      genotypically belonged to an American strain, but shared high homology with JXA1, 
      the highly pathogenic strain endemic to China. All of the 11 PRRSV isolates 
      demonstrated a 90-nucleotide deletion in the NSP2 gene, suggesting that the main 
      epidemic of PRRSV in 2008 was due to this gene deletion isolate. More consistent 
      mutations were found in specific regions of the ORF5 and ORF7 genes of the 11 
      PRRSV isolates, such as the signal peptide and transmembrane regions of GP5 and 
      the Pat 7 motif of the N protein. Whether these mutations influence nuclear 
      localization of PRRSV requires confirmation by future studies.
FAU - Li, Jida
AU  - Li J
AD  - College of Animal Science and Veterinary Medicine, Qingdao Agricultural 
      University, Qingdao 266109, China.
FAU - Yin, Yanbo
AU  - Yin Y
FAU - Guo, Baoqing
AU  - Guo B
FAU - Zhou, Shun
AU  - Zhou S
FAU - Zhang, Yi
AU  - Zhang Y
FAU - Liu, Xingcai
AU  - Liu X
FAU - Sun, Tingting
AU  - Sun T
LA  - eng
SI  - GENBANK/GQ466006
SI  - GENBANK/GQ466007
SI  - GENBANK/GQ466008
SI  - GENBANK/GQ466009
SI  - GENBANK/GQ466010
SI  - GENBANK/GQ466011
SI  - GENBANK/GQ466012
SI  - GENBANK/GQ466013
SI  - GENBANK/GQ466014
SI  - GENBANK/GQ466015
SI  - GENBANK/GQ466016
SI  - GENBANK/HM053556
SI  - GENBANK/HM053557
SI  - GENBANK/HM053558
SI  - GENBANK/HM053559
SI  - GENBANK/HM053560
SI  - GENBANK/HM053561
SI  - GENBANK/HM053562
SI  - GENBANK/HM053563
SI  - GENBANK/HM053564
SI  - GENBANK/HM053565
SI  - GENBANK/HM053566
SI  - GENBANK/HM053567
SI  - GENBANK/JN216892
SI  - GENBANK/JN216893
SI  - GENBANK/JN216894
SI  - GENBANK/JN216895
SI  - GENBANK/JN216896
SI  - GENBANK/JN216897
SI  - GENBANK/JN216898
SI  - GENBANK/JN216899
SI  - GENBANK/JN216900
SI  - GENBANK/JN216901
PT  - Journal Article
DEP - 20120530
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - China
MH  - Cluster Analysis
MH  - Genotype
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*classification/*genetics/isolation & purification
MH  - RNA, Viral/*genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Deletion
MH  - Sequence Homology, Amino Acid
MH  - Swine
MH  - Viral Proteins/*genetics
EDAT- 2012/05/31 06:00
MHDA- 2013/03/23 06:00
CRDT- 2012/05/31 06:00
PHST- 2011/07/14 00:00 [received]
PHST- 2012/05/14 00:00 [accepted]
PHST- 2012/05/31 06:00 [entrez]
PHST- 2012/05/31 06:00 [pubmed]
PHST- 2013/03/23 06:00 [medline]
AID - 10.1007/s11262-012-0763-4 [doi]
PST - ppublish
SO  - Virus Genes. 2012 Oct;45(2):256-64. doi: 10.1007/s11262-012-0763-4. Epub 2012 May 
      30.