PMID- 22645015
OWN - NLM
STAT- MEDLINE
DCOM- 20130207
LR  - 20211021
IS  - 1432-0991 (Electronic)
IS  - 0343-8651 (Linking)
VI  - 65
IP  - 3
DP  - 2012 Sep
TI  - Effects of xylitol on xylitol-sensitive versus xylitol-resistant Streptococcus 
      mutans strains in a three-species in vitro biofilm.
PG  - 237-43
LID - 10.1007/s00284-012-0151-2 [doi]
AB  - We studied the effects of xylitol on biofilms containing xylitol-resistant (Xr) 
      and xylitol-sensitive (Xs) Streptococcus mutans, Actinomyces naeslundii and S. 
      sanguinis. The biofilms were grown for 8 and 24 h on hydroxyapatite discs. The 
      viable microorganisms were determined by plate culturing techniques and 
      fluorescence in situ hybridization (FISH) was performed using a S. 
      mutans-specific probe. Extracellular cell-bound polysaccharides (EPS) were 
      determined by spectrofluorometry from single-species S. mutans biofilms. In the 
      presence of 5 % xylitol, the counts of the Xs S. mutans decreased tenfold in the 
      young (8 h) biofilm (p < 0.05) but no effect was seen in the mature (24 h) 
      biofilm. No decrease was observed for the Xr strains, and FISH confirmed these 
      results. No differences were detected in the EPS production of the Xs S. mutans 
      grown with or without xylitol, nor between Xr and Xs S. mutans strains. Thus, it 
      seems that xylitol did not affect the EPS synthesis of the S. mutans strains. 
      Since the Xr S. mutans strains, not inhibited by xylitol, showed no 
      xylitol-induced decrease in the biofilms, we conclude that growth inhibition 
      could be responsible for the decrease of the counts of the Xs S. mutans strains 
      in the clinically relevant young biofilms.
FAU - Marttinen, Aino M
AU  - Marttinen AM
AD  - Institute of Dentistry, University of Turku, Lemminkaisenkatu 2, 20520 Turku, 
      Finland.
FAU - Ruas-Madiedo, Patricia
AU  - Ruas-Madiedo P
FAU - Hidalgo-Cantabrana, Claudio
AU  - Hidalgo-Cantabrana C
FAU - Saari, Markku A
AU  - Saari MA
FAU - Ihalin, Riikka A
AU  - Ihalin RA
FAU - Soderling, Eva M
AU  - Soderling EM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120530
PL  - United States
TA  - Curr Microbiol
JT  - Current microbiology
JID - 7808448
RN  - 0 (Anti-Bacterial Agents)
RN  - VCQ006KQ1E (Xylitol)
SB  - IM
MH  - Analysis of Variance
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Biofilms/*drug effects/growth & development
MH  - Colony Count, Microbial
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Saliva/microbiology
MH  - Streptococcus mutans/*drug effects/physiology
MH  - Xylitol/*pharmacology
EDAT- 2012/05/31 06:00
MHDA- 2013/02/08 06:00
CRDT- 2012/05/31 06:00
PHST- 2012/01/18 00:00 [received]
PHST- 2012/05/12 00:00 [accepted]
PHST- 2012/05/31 06:00 [entrez]
PHST- 2012/05/31 06:00 [pubmed]
PHST- 2013/02/08 06:00 [medline]
AID - 10.1007/s00284-012-0151-2 [doi]
PST - ppublish
SO  - Curr Microbiol. 2012 Sep;65(3):237-43. doi: 10.1007/s00284-012-0151-2. Epub 2012 
      May 30.