PMID- 22647375
OWN - NLM
STAT- MEDLINE
DCOM- 20121026
LR  - 20211021
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 93
IP  - Pt 9
DP  - 2012 Sep
TI  - Natural interspecies recombinant bovine/porcine enterovirus in sheep.
PG  - 1941-1951
LID - 10.1099/vir.0.041335-0 [doi]
AB  - Members of the genus Enterovirus (family Picornaviridae) are believed to be 
      common and widespread among humans and different animal species, although only a 
      few enteroviruses have been identified from animal sources. Intraspecies 
      recombination among human enteroviruses is a well-known phenomenon, but only a 
      few interspecies examples have been reported and, to our current knowledge, none 
      of these have involved non-primate enteroviruses. In this study, we report the 
      detection and complete genome characterization (using RT-PCR and long-range PCR) 
      of a natural interspecies recombinant bovine/porcine enterovirus (ovine 
      enterovirus type 1; OEV-1) in seven (44 %) of 16 faecal samples from 3-week-old 
      domestic sheep (Ovis aries) collected in two consecutive years. Phylogenetic 
      analysis of the complete coding region revealed that OEV-1 
      (ovine/TB4-OEV/2009/HUN; GenBank accession no. JQ277724) was a novel member of 
      the species Porcine enterovirus B (PEV-B), implying the endemic presence of PEV-B 
      viruses among sheep. However, the 5' UTR of OEV-1 showed a high degree of 
      sequence and structural identity to bovine enteroviruses. The presumed 
      recombination breakpoint was mapped to the end of the 5' UTR at nucleotide 
      position 814 using sequence and SimPlot analyses. The interspecies-recombinant 
      nature of OEV-1 suggests a closer relationship among bovine and porcine 
      enteroviruses, enabling the exchange of at least some modular genetic elements 
      that may evolve independently.
FAU - Boros, Akos
AU  - Boros A
AD  - Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric 
      Viruses, ANTSZ Regional Institute of State Public Health Service, H-7623, 
      Szabadsag ut 7, Pecs, Hungary.
FAU - Pankovics, Peter
AU  - Pankovics P
AD  - Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric 
      Viruses, ANTSZ Regional Institute of State Public Health Service, H-7623, 
      Szabadsag ut 7, Pecs, Hungary.
FAU - Knowles, Nick J
AU  - Knowles NJ
AD  - Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, 
      Surrey, GU24 0NF, UK.
FAU - Reuter, Gabor
AU  - Reuter G
AD  - Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric 
      Viruses, ANTSZ Regional Institute of State Public Health Service, H-7623, 
      Szabadsag ut 7, Pecs, Hungary.
LA  - eng
SI  - GENBANK/JQ277724
SI  - GENBANK/JQ277725
SI  - GENBANK/JQ277726
GR  - BBS/E/I/00001443/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120530
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Base Sequence
MH  - Enterovirus/chemistry/classification/genetics/isolation & purification
MH  - Enterovirus Infections/*veterinary/virology
MH  - Enterovirus, Bovine/chemistry/classification/*genetics/isolation & purification
MH  - Enteroviruses, Porcine/chemistry/classification/*genetics/isolation & 
      purification
MH  - Feces/virology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Phylogeny
MH  - RNA, Viral/chemistry/genetics
MH  - *Recombination, Genetic
MH  - Sheep
MH  - Sheep Diseases/*virology
MH  - Sheep, Domestic
EDAT- 2012/06/01 06:00
MHDA- 2012/10/27 06:00
CRDT- 2012/06/01 06:00
PHST- 2012/06/01 06:00 [entrez]
PHST- 2012/06/01 06:00 [pubmed]
PHST- 2012/10/27 06:00 [medline]
AID - 10.1099/vir.0.041335-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2012 Sep;93(Pt 9):1941-1951. doi: 10.1099/vir.0.041335-0. Epub 2012 
      May 30.