PMID- 22648000
OWN - NLM
STAT- MEDLINE
DCOM- 20130813
LR  - 20211021
IS  - 1433-2965 (Electronic)
IS  - 0937-941X (Linking)
VI  - 24
IP  - 3
DP  - 2013 Mar
TI  - Treatment of hydrogen molecule abates oxidative stress and alleviates bone loss 
      induced by modeled microgravity in rats.
PG  - 969-78
LID - 10.1007/s00198-012-2028-4 [doi]
AB  - Treatment with molecular hydrogen alleviates microgravity-induced bone loss 
      through abating oxidative stress, restoring osteoblastic differentiation, and 
      suppressing osteoclast differentiation and osteoclastogenesis. INTRODUCTION: 
      Recently, it has been suggested that hydrogen gas exerts a therapeutic 
      antioxidant activity by selectively reducing cytotoxic reactive oxygen species 
      (ROS). The aim of the present study was to elucidate whether treatment with 
      molecular hydrogen alleviated bone loss induced by modeled microgravity in rats. 
      METHODS: Hindlimb suspension (HLS) and rotary wall vessel bioreactor were used to 
      model microgravity in vivo and in vitro, respectively. Sprague-Dawley rats were 
      exposed to HLS for 6 weeks to induced bone loss and simultaneously administrated 
      with hydrogen water (HW). Then, we investigated the effects of incubation with 
      hydrogen-rich medium (HRM) on MC3T3-E1 and RAW264.7 cells exposed to modeled 
      microgravity. RESULTS: Treatment with HW alleviated HLS-induced reduction of bone 
      mineral density, ultimate load, stiffness, and energy in femur and lumbar 
      vertebra. Treatment with HW alleviated HLS-induced augmentation of 
      malondialdehyde content and peroxynitrite content and reduction of total 
      sulfhydryl content in femur and lumbar vertebra. In cultured MC3T3-E1 cells, 
      incubation with HRM inhibited modeled microgravity-induced ROS formation, 
      reduction of osteoblastic differentiation, increase of ratio of receptor 
      activator of nuclear factor kappa B ligand to osteoprotegerin, inducible nitric 
      oxide synthetase upregulation, and Erk1/2 phosphorylation. In cultured RAW264.7, 
      incubation with HRM aggravated modeled microgravity-induced ROS formation, 
      osteoclastic differentiation, and osteoclastogenesis. CONCLUSION: Treatment with 
      molecular hydrogen alleviates microgravity-induced bone loss in rats. Molecular 
      hydrogen could thus be envisaged as a nutritional countermeasure for spaceflight 
      but remains to be tested in humans.
FAU - Sun, Y
AU  - Sun Y
AD  - Department of Emergency, The Military General Hospital of Beijing PLA, Beijing 
      100700, China.
FAU - Shuang, F
AU  - Shuang F
FAU - Chen, D M
AU  - Chen DM
FAU - Zhou, R B
AU  - Zhou RB
LA  - eng
PT  - Journal Article
DEP - 20120531
PL  - England
TA  - Osteoporos Int
JT  - Osteoporosis international : a journal established as result of cooperation 
      between the European Foundation for Osteoporosis and the National Osteoporosis 
      Foundation of the USA
JID - 9100105
RN  - 0 (Antioxidants)
RN  - 0 (Bone Density Conservation Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 059QF0KO0R (Water)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology/*therapeutic use
MH  - Bone Density/drug effects
MH  - Bone Density Conservation Agents/pharmacology/*therapeutic use
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Femur/physiopathology
MH  - Hindlimb Suspension
MH  - Hydrogen/pharmacology/*therapeutic use
MH  - Lumbar Vertebrae/physiopathology
MH  - Osteoblasts/drug effects/pathology
MH  - Osteoclasts/drug effects/pathology
MH  - Osteoporosis/etiology/physiopathology/*prevention & control
MH  - Oxidative Stress/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - Water/chemistry
MH  - *Weightlessness
EDAT- 2012/06/01 06:00
MHDA- 2013/08/14 06:00
CRDT- 2012/06/01 06:00
PHST- 2012/03/19 00:00 [received]
PHST- 2012/05/14 00:00 [accepted]
PHST- 2012/06/01 06:00 [entrez]
PHST- 2012/06/01 06:00 [pubmed]
PHST- 2013/08/14 06:00 [medline]
AID - 10.1007/s00198-012-2028-4 [doi]
PST - ppublish
SO  - Osteoporos Int. 2013 Mar;24(3):969-78. doi: 10.1007/s00198-012-2028-4. Epub 2012 
      May 31.