PMID- 22648044
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20120727
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 148
IP  - 1-2
DP  - 2012 Jul 15
TI  - Effect of the host cell line on the vaccine efficacy of an attenuated porcine 
      reproductive and respiratory syndrome virus.
PG  - 116-25
LID - 10.1016/j.vetimm.2012.05.008 [doi]
AB  - The abilities of the modified-live Prime Pac (PP) strain of porcine reproductive 
      and respiratory syndrome virus (PRRSV), propagated in either traditional simian 
      cells (MARC-145) or in a novel porcine alveolar macrophage cell line (ZMAC), to 
      confer pigs protection against subsequent PRRSV challenge were compared. Eight 
      week-old pigs were injected with PP virus grown in one of the two cell types and 
      then exposed 4 weeks later to the "atypical" PRRSV isolate NADC-20. Control 
      animals were similarly challenged or remained PRRSV-naive. While the average 
      adjusted body weight (aabw) of the strict control group increased 22% by 10 days 
      post challenge (pc), this value for the non-vaccinated, challenged group dropped 
      4%. In contrast, prior immunization with PP virus, regardless of its host cell 
      source, ameliorated this effect by affording a >9% rise in aabw. Likewise, nearly 
      equivalent protection was extended to both groups of vaccinates in regards to the 
      temporal elimination of their pc clinical distress and viremia. However, the PP 
      virus propagated in ZMAC cells appeared to be more efficacious since four of the 
      six pigs receiving this biologic cleared the challenge virus from the their lungs 
      by 10 days pc as compared to only one member of the other vaccinated group. 
      Notably, the predominant quasispecies in the ZMAC cell-prepared PP virus stock 
      contained a highly conserved N-glycosylation site at position 184 in its 
      glycoprotein 2 while this entity was underrepresented in the MARC-145 cell grown 
      biologic. Since glycoprotein 2 is involved in infectivity, such additional 
      glycosylation may enhance virus replication in porcine alveolar macrophages.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Calzada-Nova, Gabriela
AU  - Calzada-Nova G
AD  - Department of Pathobiology, College of Veterinary Medicine, University of 
      Illinois, 2001 South Lincoln Avenue, Urbana, IL, USA.
FAU - Husmann, Robert J
AU  - Husmann RJ
FAU - Schnitzlein, William M
AU  - Schnitzlein WM
FAU - Zuckermann, Federico A
AU  - Zuckermann FA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120511
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (RNA, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Body Weight/immunology
MH  - Bronchoalveolar Lavage Fluid/virology
MH  - Immunization/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - RNA, Viral/chemistry/genetics
MH  - Random Allocation
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Specific Pathogen-Free Organisms
MH  - Swine
MH  - Vaccines, Attenuated/immunology/pharmacology
MH  - Viral Load/veterinary
MH  - Viral Vaccines/immunology/*pharmacology
MH  - Viremia/immunology/prevention & control/*veterinary/virology
EDAT- 2012/06/01 06:00
MHDA- 2013/01/25 06:00
CRDT- 2012/06/01 06:00
PHST- 2011/02/18 00:00 [received]
PHST- 2012/04/17 00:00 [revised]
PHST- 2012/05/04 00:00 [accepted]
PHST- 2012/06/01 06:00 [entrez]
PHST- 2012/06/01 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
AID - S0165-2427(12)00156-0 [pii]
AID - 10.1016/j.vetimm.2012.05.008 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2012 Jul 15;148(1-2):116-25. doi: 
      10.1016/j.vetimm.2012.05.008. Epub 2012 May 11.