PMID- 22648284
OWN - NLM
STAT- MEDLINE
DCOM- 20130311
LR  - 20220311
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 29
IP  - 9
DP  - 2012 Sep
TI  - Efficient combined FISH and PRINS technique for detection of DAZ microdeletion in 
      human sperm.
PG  - 979-84
LID - 10.1007/s10815-012-9805-z [doi]
AB  - Intracytoplasmic sperm injection (ICSI) now offers an effective therapeutic 
      option for men with male infertility and is believed to allow transmission of 
      genetically determined infertility to the male offspring. Transmission of DAZ 
      (Deleted in Azoospermia) microdeletion is one of the major concerns for oligo and 
      severe oligozoospermia patients. Screening of the Y chromosome microdeletion in 
      the diagnostic work-up of infertile men is mainly done using polymerase chain 
      reaction (PCR) on blood leukocytes. However, there are evidences showing that 
      presence of DAZ in somatic cells might not be indicative of its presence in germ 
      cell lineage. In this report we are going to describe a combined Primed in situ 
      labeling (PRINS) and fluorescence in situ hybridization (FISH) technique to show 
      the localization of DAZ gene as well as Y chromosome centromere on sperm nuclei. 
      PRINS is a combination of FISH and in situ polymerization provides another 
      approach for in situ chromosomal detection. In the present study the PRINS 
      primers specific for DAZ genes and traditional direct labeled centromere FISH 
      probes for Y and X chromosomes were used in order to simultaneously detect DAZ 
      genes and sex chromosome aneuploidy in sperm samples.
FAU - Mozdarani, Hossein
AU  - Mozdarani H
AD  - Department of Medical Genetics, Faculty of Medical Sciences , Tarbiat Modares 
      University, Tehran, Iran. mozdarah@modares.ac.ir
FAU - Ghoraeian, Pegah
AU  - Ghoraeian P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120531
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (DAZ1 protein, human)
RN  - 0 (DNA Probes)
RN  - 0 (Deleted in Azoospermia 1 Protein)
RN  - 0 (RNA-Binding Proteins)
RN  - Male sterility due to Y-chromosome deletions
SB  - IM
MH  - Adult
MH  - Cell Nucleus/genetics
MH  - Centromere/genetics
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Y/genetics
MH  - DNA Probes
MH  - Deleted in Azoospermia 1 Protein
MH  - Genetic Testing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infertility, Male
MH  - Male
MH  - Oligospermia/genetics
MH  - Primed In Situ Labeling/*methods
MH  - RNA-Binding Proteins/*genetics
MH  - Reproducibility of Results
MH  - Semen Analysis/methods
MH  - Sensitivity and Specificity
MH  - Sex Chromosome Aberrations
MH  - Sex Chromosome Disorders of Sex Development/*genetics
MH  - Sperm Count
MH  - Spermatozoa/*cytology
PMC - PMC3463660
EDAT- 2012/06/01 06:00
MHDA- 2013/03/12 06:00
PMCR- 2013/09/01
CRDT- 2012/06/01 06:00
PHST- 2012/02/22 00:00 [received]
PHST- 2012/05/20 00:00 [accepted]
PHST- 2012/06/01 06:00 [entrez]
PHST- 2012/06/01 06:00 [pubmed]
PHST- 2013/03/12 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - 9805 [pii]
AID - 10.1007/s10815-012-9805-z [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2012 Sep;29(9):979-84. doi: 10.1007/s10815-012-9805-z. 
      Epub 2012 May 31.