PMID- 22648708
OWN - NLM
STAT- MEDLINE
DCOM- 20121001
LR  - 20211021
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 461
IP  - 1
DP  - 2012 Jul
TI  - FGFR1 expression and gene copy numbers in human lung cancer.
PG  - 49-57
LID - 10.1007/s00428-012-1250-y [doi]
AB  - FGFR1 is a receptor tyrosine kinase of which the ligands belong to the fibroblast 
      growth factor family. To evaluate the significance of FGFR1 in lung cancer, we 
      analysed tumours by immunohistochemistry (IHC) and fluorescence in situ 
      hybridization (FISH). Tissue microarrays were constructed containing 380 lung 
      cancer samples including squamous cell carcinomas (SCC), adenocarcinomas (ADC), 
      non-small cell lung cancer not otherwise specified, metastases, neuroendocrine 
      tumours, large cell lung cancer and small cell lung cancer. FGFR1 expression was 
      analysed by IHC and scored semi-quantitatively by a four-tier approach (0, 1, 2, 
      3). Using dual-colour interphase FISH with probes specific for the locus on 8p12 
      and the centromere of chromosome 8 (CEN8), copy numbers of FGFR1 were determined. 
      High expression of FGFR1 was associated with increased FGFR1 gene copy numbers in 
      squamous cell carcinoma (p < 0.001). The FGFR1 locus was equally affected by copy 
      number losses and gains. The higher FGFR1 gene copy numbers in SCC compared to 
      ADC did not reach statistical significance. High copy number amplification of 
      FGFR1 was a very rare event, the FGFR1/CEN8 signal ratio reaching a maximum value 
      of 2.75. There were no significant associations between FGFR1 and 
      clinicopathological parameters. Fibroblast growth factor signalling represents an 
      interesting therapeutic target in lung cancer. However, the pathways are complex 
      with potential oncogenic and anti-oncogenic activities. Our data may help to 
      define criteria for selecting patients that may benefit from these new 
      therapeutic options.
FAU - Kohler, Lukas H
AU  - Kohler LH
AD  - Institute of Pathology, Jena University Hospital, Friedrich-Schiller-University, 
      Ziegelmuehlenweg 1, 07740 Jena, Germany.
FAU - Mireskandari, Masoud
AU  - Mireskandari M
FAU - Knosel, Thomas
AU  - Knosel T
FAU - Altendorf-Hofmann, Annelore
AU  - Altendorf-Hofmann A
FAU - Kunze, Almut
AU  - Kunze A
FAU - Schmidt, Andreas
AU  - Schmidt A
FAU - Presselt, Norbert
AU  - Presselt N
FAU - Chen, Yuan
AU  - Chen Y
FAU - Petersen, Iver
AU  - Petersen I
LA  - eng
PT  - Journal Article
DEP - 20120531
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - EC 2.7.10.1 (FGFR1 protein, human)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 1)
SB  - IM
MH  - Aged
MH  - Female
MH  - *Gene Dosage
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Kaplan-Meier Estimate
MH  - Lung Neoplasms/*genetics/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Neoplasm Staging
MH  - Receptor, Fibroblast Growth Factor, Type 1/*biosynthesis/*genetics
MH  - Tissue Array Analysis
EDAT- 2012/06/01 06:00
MHDA- 2012/10/02 06:00
CRDT- 2012/06/01 06:00
PHST- 2012/03/03 00:00 [received]
PHST- 2012/05/10 00:00 [accepted]
PHST- 2012/04/05 00:00 [revised]
PHST- 2012/06/01 06:00 [entrez]
PHST- 2012/06/01 06:00 [pubmed]
PHST- 2012/10/02 06:00 [medline]
AID - 10.1007/s00428-012-1250-y [doi]
PST - ppublish
SO  - Virchows Arch. 2012 Jul;461(1):49-57. doi: 10.1007/s00428-012-1250-y. Epub 2012 
      May 31.