PMID- 22650038
OWN - NLM
STAT- MEDLINE
DCOM- 20130530
LR  - 20120601
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 43
IP  - 2
DP  - 2012 Mar
TI  - [Implication of BDNF expression in transected spinal cord of rats].
PG  - 231-5
AB  - OBJECTIVE: To explore the change of brain derived neurotrophic factor (BDNF) 
      expression and its relationship with the neurological behavior after spinal cord 
      transection (SCT) in rats. METHODS: 66 adult SD rats were assigned randomly to 
      sham operation group and SCT group. Rats in SCT group were subjected T10-T11 
      spinal cord transection and allowed to survived 1 d, 3 d, 7 d, 14 d, 21 d and 28 
      d. BBB scores in hindlimbs were observed at 0, 7, 14, 21 and 28 days post 
      operation (dpo). The BDNF expression was determined by using ELISA (28 d) and 
      RT-PCR (each time point) techniques. The localization of BDNF and its mRNA at 
      sham and 28 d after SCT was also observed by immunohistochemistry and in situ 
      hybridization. RESULTS: After SCT, the motor function in hindlimbs disappear 
      immediately. The BBB scores get a gradual recovery from 14 dpo to 28 dpo, when 
      compared with that of former timepoint (P < 0.05). BDNF and its mRNA were located 
      in cytoplasma and neurites. The level of BDNF mRNA (indicated by RT-PCR) was 
      upregulated at 14 days after SCT than that in 1 and 3 dpo (P< 0. 05). The 
      contents of BDNF in the injuried spinal cords at 28 dpo were increased than that 
      in the sham operation group (P < 0.05). CONCLUSION: Neuroplasticity has occurred 
      in rats subjected to SCT, and the mechanism may be involved in the increase of 
      BDNF expression in the spinal cord with the time going.
FAU - Jin, Huan
AU  - Jin H
AD  - Department of Anesthesiology, the First Affiliated Hospital of Yunnan Province, 
      Kunhua Hospital, Kunming Medical College, Kunming 650031, China.
FAU - Dan, Qi-Qin
AU  - Dan QQ
FAU - Rong, Rong
AU  - Rong R
FAU - Wang, Ting-Hua
AU  - Wang TH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Male
MH  - Neuronal Plasticity/physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord Injuries/*metabolism
EDAT- 2012/06/02 06:00
MHDA- 2013/06/01 06:00
CRDT- 2012/06/02 06:00
PHST- 2012/06/02 06:00 [entrez]
PHST- 2012/06/02 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 Mar;43(2):231-5.