PMID- 22650041
OWN - NLM
STAT- MEDLINE
DCOM- 20130530
LR  - 20131121
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 43
IP  - 2
DP  - 2012 Mar
TI  - [Effect of methylprednisolone on neurological behavior and the BDNF and NMDA 
      receptor expression after traumatic spinal cord injury in rats].
PG  - 245-9
AB  - OBJECTIVE: To study the effect of methylprednisolone on spinal cord injury rats' 
      neural behavior and the expression of brain derived neurotrophic factor (BDNF) 
      and N-methyl-D-aspartic acid (NMDA). METHODS: To establish rat model of spinal 
      cord injury (SCI), the rats were randomly divided into sham operation group, SCI 
      group and methylprednisolone group (n = 16 in each group). Eight rats were used 
      for the behavioral assessment and BDNF measurement,the other eight animals was 
      for the NMDA receptor test in each group. Within 8 h spinal cord contusion, 
      methylprednisolone (50 mg/kg) was injected for methylprednisolone group, then 
      after that the intramuscular injection of methylprednisolone was per day 
      reduction in 10 mg/kg, till 5 days. By using immunohistochemical staining, the 
      distribution of BDNF in the spinal cord and positive cell localization was 
      observed and the number of positive cells were counted. The NMDA receptor 
      affinity (Kd) and maximum binding amount (Bmax) were measured with [3H] MK-801 
      radioligand method, and the rat hind limb functional was also evaluated with BBB 
      score analysis. RESULTS: Both the number of BDNF positive cells and the BBB score 
      in methylprednisolone group was significant higher than that of SCI group; While 
      increased receptor affinity (Kd) and decreased Bmax for NMDA receptor in 
      methylprednisolone group was seen less than in SCI group (P < 0.05). CONCLUSION: 
      Methylprednisolone can improve the function of rat hind limb, increase BDNF level 
      and decreased NMDA receptor expression after spinal cord injury.
FAU - Yang, Jun
AU  - Yang J
AD  - Department of Bone Injury, Yuxi Municipal Hospital of Traditional Chinese 
      Medicine, Yuxi 653100, China.
FAU - Xu, Zheng-Guang
AU  - Xu ZG
FAU - Rong, Rong
AU  - Rong R
FAU - Lu, Zhi-Ping
AU  - Lu ZP
FAU - Sun, Yan-Hua
AU  - Sun YH
FAU - Zhao, Ya
AU  - Zhao Y
FAU - Li, Xiao-Song
AU  - Li XS
FAU - Liu, Jiang-Hua
AU  - Liu JH
FAU - Dan, Qi-Qin
AU  - Dan QQ
FAU - Zhao, Nan
AU  - Zhao N
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Male
MH  - Methylprednisolone/*therapeutic use
MH  - Neurons/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/genetics/*metabolism
MH  - Recovery of Function/*drug effects
MH  - Spinal Cord Injuries/*drug therapy/metabolism
EDAT- 2012/06/02 06:00
MHDA- 2013/06/01 06:00
CRDT- 2012/06/02 06:00
PHST- 2012/06/02 06:00 [entrez]
PHST- 2012/06/02 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 Mar;43(2):245-9.