PMID- 22650044
OWN - NLM
STAT- MEDLINE
DCOM- 20130530
LR  - 20120601
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 43
IP  - 2
DP  - 2012 Mar
TI  - [Constrution of human single herpes virus carried BDNF gene vector and its 
      bioactivity evaluation].
PG  - 258-61
AB  - OBJECTIVE: To construct the recombinant vector of human single herpes virus (HSV) 
      carried brain derived neurotrophic factor (BDNF) gene. METHODS: BDNF gene was 
      acquired from rat brain tissue by RT-PCR, then was cloned into plasmid, and 
      enveloped by HSV. The recombinant was used to transfer cultured cortical neurons. 
      The number and neurite length of neurons were quantified. The BDNF level and 
      subcellular localization were detected by ELISA and immunohistochemistry. 
      RESULTS: HSV carried BDNF gene recombinant has been successfully constructed. The 
      recombinant showed the bioactivity on the growth of cortical neurons. BDNF level 
      was increased significantly in BDNF transferred group. CONCLUSION: HSV carried 
      BDNF gene recombinant, with the bioactivity, has been successfully constructed. 
      This could provide the vector for the treatment of BDNF under disease condition 
      base on transferring gene technique.
FAU - Wang, Sheng-Lan
AU  - Wang SL
AD  - Department of Resparation, the First Affiliated Hospital of Yunnan Province, 
      Kunhua Hospital, Kunming Medical College, Kunming 650031, China.
FAU - Dan, Qi-Qin
AU  - Dan QQ
FAU - Rong, Rong
AU  - Rong R
FAU - Wang, Ting-Hua
AU  - Wang TH
FAU - Zhang, Yun-Hui
AU  - Zhang YH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/*metabolism
MH  - Cloning, Molecular
MH  - *Genetic Vectors
MH  - Herpesvirus 1, Human/genetics/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neurons/cytology/metabolism
MH  - Rats
MH  - Recombinant Proteins/biosynthesis/genetics
MH  - *Transfection
EDAT- 2012/06/02 06:00
MHDA- 2013/06/01 06:00
CRDT- 2012/06/02 06:00
PHST- 2012/06/02 06:00 [entrez]
PHST- 2012/06/02 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 Mar;43(2):258-61.