PMID- 22650045
OWN - NLM
STAT- MEDLINE
DCOM- 20130530
LR  - 20120601
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 43
IP  - 2
DP  - 2012 Mar
TI  - [Experiment study on the treatment of SCI based on HSV carried BDNF transgene 
      technique].
PG  - 262-5
AB  - OBJECTIVE: To explore the effect of brain derived neurotrophic factor (BDNF) 
      transgenic treatment in rats following spinal cord injury (SCI). METHODS: BDNF 
      gene was cloned into plasmid then enveloped with human single herpes virus (HSV) 
      to construct HSV carried BDNF transgenic recombinant. BDNF recombinant was 
      injected into sciatic nerve to last label in motorneurous in the caudal cords, 
      then ventral motor neurons were counted and the area of cell body was measured. 
      The BBB scores representing motor function in hindlimbs was also recorded. 
      RESULTS: Five days were needed for the GFP-HSV to arrive motorneurons from 
      sciatic nerve. BDNF release could increase the number of motroneurons and inhibit 
      neuronal atrophy in injured spinal cord. BDNF administration also improves motor 
      function in hindlimbs. CONCLUSION: BDNF transgene carried by HSV is a useful 
      strategy for the treatment of SCI, indicating its clinic implication in future 
      treatment.
FAU - Rong, Rong
AU  - Rong R
AD  - Cadre's Ward, Anhui Provincial Hospital, Hefei 230001, China.
FAU - Dan, Qi-Qin
AU  - Dan QQ
FAU - Liu, Jia
AU  - Liu J
FAU - Hu, Li-Qun
AU  - Hu LQ
FAU - Wang, Ting-Hua
AU  - Wang TH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Cloning, Molecular
MH  - Genetic Therapy/methods
MH  - Genetic Vectors
MH  - Herpesvirus 1, Human/genetics/*metabolism
MH  - Male
MH  - Microinjections
MH  - *Nerve Regeneration
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recombinant Proteins/*administration & dosage/biosynthesis/genetics
MH  - Sciatic Nerve
MH  - Spinal Cord Injuries/metabolism/*therapy
MH  - Transfection
EDAT- 2012/06/02 06:00
MHDA- 2013/06/01 06:00
CRDT- 2012/06/02 06:00
PHST- 2012/06/02 06:00 [entrez]
PHST- 2012/06/02 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 Mar;43(2):262-5.