PMID- 22651102
OWN - NLM
STAT- MEDLINE
DCOM- 20130104
LR  - 20240322
IS  - 1423-0127 (Electronic)
IS  - 1021-7770 (Print)
IS  - 1021-7770 (Linking)
VI  - 19
IP  - 1
DP  - 2012 May 31
TI  - The effect of PTZ-induced epileptic seizures on hippocampal expression of 
      PSA-NCAM in offspring born to kindled rats.
PG  - 56
LID - 10.1186/1423-0127-19-56 [doi]
AB  - BACKGROUND: Maternal epileptic seizures during pregnancy can affect the 
      hippocampal neurons in the offspring. The polysialylated neural cell adhesion 
      molecule (PSA-NCAM), which is expressed in the developing central nervous system, 
      may play important roles in neuronal migration, synaptogenesis, and axonal 
      outgrowth. This study was designed to assess the effects of kindling either with 
      or without maternal seizures on hippocampal PSA-NCAM expression in rat offspring. 
      METHODS: Forty timed-pregnant Wistar rats were divided into four groups: A) 
      Kind+/Seiz+, pregnant kindled (induced two weeks prior to pregnancy) rats that 
      received repeated intraperitoneal (i.p.) pentylenetetrazol, PTZ injections on 
      gestational days (GD) 14-19; B) Kind-/Seiz+, pregnant non-kindled rats that 
      received PTZ injections on GD14-GD19; C) Kind+/Seiz-, pregnant kindled rats that 
      did not receive any PTZ injections; and D) Kind-/Seiz-, the sham controls. 
      Following birth, the pups were sacrificed on PD1 and PD14, and PSA-NCAM 
      expression and localization in neonates' hippocampi were analyzed by Western 
      blots and immunohistochemistry. RESULTS: Our data show a significant down 
      regulation of hippocampal PSA-NCAM expression in the offspring of Kind+/Seiz+ 
      (p = 0.001) and Kind-/Seiz+ (p = 0.001) groups compared to the sham control 
      group. The PSA-NCAM immunoreactivity was markedly decreased in all parts of the 
      hippocampus, especially in the CA3 region, in Kind+/Seiz+ (p = 0.007) and 
      Kind-/Seiz+ (p = 0.007) group's newborns on both PD1 and 14. CONCLUSION: Our 
      findings demonstrate that maternal seizures but not kindling influence the 
      expression of PSA-NCAM in the offspring's hippocampi, which may be considered as 
      a factor for learning/memory and cognitive impairments reported in children born 
      to epileptic mothers.
FAU - Rajabzadeh, Aliakbar
AU  - Rajabzadeh A
AD  - Department of Anatomy and Cell Biology, Mashhad, Iran.
FAU - Bideskan, Alireza Ebrahimzadeh
AU  - Bideskan AE
FAU - Fazel, Alireza
AU  - Fazel A
FAU - Sankian, Mojtaba
AU  - Sankian M
FAU - Rafatpanah, Houshang
AU  - Rafatpanah H
FAU - Haghir, Hossein
AU  - Haghir H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120531
PL  - England
TA  - J Biomed Sci
JT  - Journal of biomedical science
JID - 9421567
RN  - 0 (Neural Cell Adhesion Molecule L1)
RN  - 0 (Sialic Acids)
RN  - 0 (polysialyl neural cell adhesion molecule)
RN  - WM5Z385K7T (Pentylenetetrazole)
SB  - IM
MH  - Animals
MH  - *Epilepsy/chemically induced/metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Hippocampus/*metabolism
MH  - Kindling, Neurologic/drug effects/metabolism
MH  - Neural Cell Adhesion Molecule L1/*metabolism
MH  - Pentylenetetrazole/toxicity
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Sialic Acids/*metabolism
PMC - PMC3586948
EDAT- 2012/06/02 06:00
MHDA- 2013/01/05 06:00
PMCR- 2012/05/31
CRDT- 2012/06/02 06:00
PHST- 2012/02/26 00:00 [received]
PHST- 2012/05/31 00:00 [accepted]
PHST- 2012/06/02 06:00 [entrez]
PHST- 2012/06/02 06:00 [pubmed]
PHST- 2013/01/05 06:00 [medline]
PHST- 2012/05/31 00:00 [pmc-release]
AID - 1423-0127-19-56 [pii]
AID - 10.1186/1423-0127-19-56 [doi]
PST - epublish
SO  - J Biomed Sci. 2012 May 31;19(1):56. doi: 10.1186/1423-0127-19-56.