PMID- 22652939
OWN - NLM
STAT- MEDLINE
DCOM- 20121127
LR  - 20161020
IS  - 1528-3976 (Electronic)
IS  - 1071-5754 (Linking)
VI  - 39
IP  - 4
DP  - 2012 Jul-Aug
TI  - Effectiveness of apoptotic factors expressed on the wounds of patients with stage 
      III pressure ulcers.
PG  - 391-6
LID - 10.1097/WON.0b013e318259c47e [doi]
AB  - PURPOSE: The purpose of this study was to explore the expression of apoptotic 
      factors in stage III pressure ulcers and explain their effects on wound healing. 
      SUBJECTS AND SETTING: The sample comprised 8 patients with stage III pressure 
      ulcers, along with 4 volunteers with normal skin who were undergoing elective 
      surgical procedures. METHODS: Eight patient tissue specimens from wound of stage 
      III pressure ulcers were obtained from hospitalized patients. Tissue specimens 
      from 4 volunteer patients were obtained during surgery. All 12 tissue specimens 
      were analyzed for presence of apoptosis-related factors including bax/bcl-2 
      (B-cell lymphoma 2), Fas/fasL (Fas ligand), and terminal deoxynucleotide 
      transferase-mediated dUTP nick end labeling (TUNEL) as well as 
      immunohistochemical analysis with Fas/FasL. RESULTS: Bax positive cells were 
      strongly expressed from surrounding zone to center zone in the specimens obtained 
      from stage III pressure ulcers, and Bcl-2 expression staining decreased 
      contrarily (P < .01). Comparison, apoptotic factors bax positive cell and 
      fas/fasL mRNA expression occurred rarely in tissue specimens from the control 
      group. Fas/fasL mRNA-positive granules were detected in epidermal cells and 
      fibroblasts, demonstrating a progressive increase from the margin of the pressure 
      ulcers progressing toward the center of the wound bed. We also found an increased 
      intensity of TUNEL staining in ulcer center as well as wound margin, compared 
      with control skin tissue. CONCLUSION: These results suggest that molecular 
      regulators of apoptosis are involved in pressure ulcer wound healing. Results 
      also suggest that differences in the expression and distribution of the apoptotic 
      related factors in stage III pressure ulcers play an important role in the 
      delayed wound healing characteristic of these complex wounds.
FAU - Jiang, Liping
AU  - Jiang L
AD  - School of Nursing, Wenzhou Medical College, Wenzhou, Zhejiang Province, People's 
      Republic of China. lpngjiang@yahoo.com
FAU - Zhang, En
AU  - Zhang E
FAU - Yang, Yeqin
AU  - Yang Y
FAU - Zhang, Chunyu
AU  - Zhang C
FAU - Fu, Xiaobing
AU  - Fu X
FAU - Xiao, Jian
AU  - Xiao J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Wound Ostomy Continence Nurs
JT  - Journal of wound, ostomy, and continence nursing : official publication of The 
      Wound, Ostomy and Continence Nurses Society
JID - 9435679
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
MH  - Adult
MH  - Apoptosis/*physiology
MH  - Fas Ligand Protein/metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Middle Aged
MH  - Pressure Ulcer/metabolism/*physiopathology
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Wound Healing/*physiology
MH  - bcl-2-Associated X Protein/metabolism
EDAT- 2012/06/02 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/06/02 06:00
PHST- 2012/06/02 06:00 [entrez]
PHST- 2012/06/02 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1097/WON.0b013e318259c47e [doi]
PST - ppublish
SO  - J Wound Ostomy Continence Nurs. 2012 Jul-Aug;39(4):391-6. doi: 
      10.1097/WON.0b013e318259c47e.