PMID- 22656886
OWN - NLM
STAT- MEDLINE
DCOM- 20121026
LR  - 20171116
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 217
IP  - 8
DP  - 2012 Aug
TI  - Dendritic cell are able to differentially recognize Sporothrix schenckii antigens 
      and promote Th1/Th17 response in vitro.
PG  - 788-94
LID - 10.1016/j.imbio.2012.04.006 [doi]
AB  - Sporotrichosis is a disease caused by the dimorphic fungus Sporothrix schenckii. 
      The main clinical manifestations occur in the skin, however the number of 
      systemic and visceral cases has increased, especially in immunocompromised 
      patients. Dendritic cells (DCs) are highly capable to recognize the fungus 
      associated data and translate it into differential T cells responses both in vivo 
      and in vitro. Although, the mechanisms involved in the interaction between DCs 
      and S. schenckii are not fully elucidated. The present study investigated the 
      phenotypic and functional changes in bone marrow dendritic cells (BMDCs) 
      stimulated in vitro with the yeast form of S. schenckii or exoantigen (ExoAg) and 
      its ability to trigger a cellular immune response in vitro. Our results 
      demonstrated that the live yeast of S. schenckii and its exoantigen, at a higher 
      dose, were able to activate BMDCs and made them capable of triggering T cell 
      responses in vitro. Whereas the yeast group promoted more pronounced IFN-gamma 
      production rather than IL-17, the Exo100 group generated similar production of 
      both cytokines. The exoantigen stimulus suggests a capability to deviate the 
      immune response from an effector Th1 to an inflammatory Th17 response. 
      Interestingly, only the Exo100 group promoted the production of IL-6 and a 
      significant increase of TGF-beta, in addition to IL-23 production. Interestingly, 
      only Exo100 group was capable to promote the production of IL-6 and a significant 
      increase on TGF-beta, in addition with IL-23 detection. Our results demonstrated the 
      plasticity of DCs in translating the data associated with the fungus S. schenckii 
      and ExoAg into differential T cell responses in vitro. The possibility of using 
      ex vivo-generated DCs as vaccinal and therapeutic tools for sporotrichosis is a 
      challenge for the future.
CI  - Copyright (c) 2012 Elsevier GmbH. All rights reserved.
FAU - Verdan, F F
AU  - Verdan FF
AD  - Department of Clinical Analyses, Faculty of Pharmaceutical Sciences, Sao Paulo 
      State University, Araraquara, Sao Paulo 14801-902, Brazil.
FAU - Faleiros, J C
AU  - Faleiros JC
FAU - Ferreira, L S
AU  - Ferreira LS
FAU - Monnazzi, L G S
AU  - Monnazzi LG
FAU - Maia, D C G
AU  - Maia DC
FAU - Tansine, A
AU  - Tansine A
FAU - Placeres, M C P
AU  - Placeres MC
FAU - Carlos, I Z
AU  - Carlos IZ
FAU - Santos-Junior, R R
AU  - Santos-Junior RR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120509
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Antigens, Fungal)
RN  - 0 (CD11c Antigen)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-23)
RN  - 0 (Interleukin-6)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Antigens, Fungal/*immunology
MH  - Bone Marrow Cells/immunology/metabolism
MH  - CD11c Antigen/immunology/metabolism
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytokines/immunology/metabolism
MH  - Dendritic Cells/*immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Host-Pathogen Interactions/immunology
MH  - Humans
MH  - Interleukin-23/immunology/metabolism
MH  - Interleukin-6/immunology/metabolism
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Sporothrix/*immunology/physiology
MH  - Sporotrichosis/*immunology/metabolism/microbiology
MH  - Th1 Cells/*immunology/metabolism
MH  - Th17 Cells/*immunology/metabolism
MH  - Transforming Growth Factor beta/immunology/metabolism
EDAT- 2012/06/05 06:00
MHDA- 2012/10/27 06:00
CRDT- 2012/06/05 06:00
PHST- 2011/06/30 00:00 [received]
PHST- 2012/04/26 00:00 [revised]
PHST- 2012/04/27 00:00 [accepted]
PHST- 2012/06/05 06:00 [entrez]
PHST- 2012/06/05 06:00 [pubmed]
PHST- 2012/10/27 06:00 [medline]
AID - S0171-2985(12)00089-7 [pii]
AID - 10.1016/j.imbio.2012.04.006 [doi]
PST - ppublish
SO  - Immunobiology. 2012 Aug;217(8):788-94. doi: 10.1016/j.imbio.2012.04.006. Epub 
      2012 May 9.