PMID- 22656960
OWN - NLM
STAT- MEDLINE
DCOM- 20121030
LR  - 20161125
IS  - 1532-1991 (Electronic)
IS  - 0143-4160 (Linking)
VI  - 52
IP  - 2
DP  - 2012 Aug
TI  - TRPV1-mediated calcium signal couples with cannabinoid receptors and 
      sodium-calcium exchangers in rat odontoblasts.
PG  - 124-36
LID - 10.1016/j.ceca.2012.05.002 [doi]
AB  - Odontoblasts are involved in the transduction of stimuli applied to exposed 
      dentin. Although expression of thermo/mechano/osmo-sensitive transient receptor 
      potential (TRP) channels has been demonstrated, the properties of TRP vanilloid 1 
      (TRPV1)-mediated signaling remain to be clarified. We investigated physiological 
      and pharmacological properties of TRPV1 and its functional coupling with 
      cannabinoid (CB) receptors and Na(+)-Ca(2+) exchangers (NCXs) in odontoblasts. 
      Anandamide (AEA), capsaicin (CAP), resiniferatoxin (RF) or low-pH evoked Ca(2+) 
      influx. This influx was inhibited by capsazepine (CPZ). Delay in 
      time-to-activation of TRPV1 channels was observed between application of AEA or 
      CAP and increase in [Ca(2+)](i). In the absence of extracellular Ca(2+), however, 
      an immediate increase in [Ca(2+)](i) was observed on administration of 
      extracellular Ca(2+), followed by activation of TRPV1 channels. Intracellular 
      application of CAP elicited inward current via opening of TRPV1 channels faster 
      than extracellular application. With extracellular RF application, no time delay 
      was observed in either increase in [Ca(2+)](i) or inward current, indicating that 
      agonist binding sites are located on both extra- and intracellular domains. 
      KB-R7943, an NCX inhibitor, yielded an increase in the decay time constant during 
      TRPV1-mediated Ca(2+) entry. Increase in [Ca(2+)](i) by CB receptor agonist, 
      2-arachidonylglycerol, was inhibited by CB1 receptor antagonist or CPZ, as well 
      as by adenylyl cyclase inhibitor. These results showed that TRPV1-mediated Ca(2+) 
      entry functionally couples with CB1 receptor activation via cAMP signaling. 
      Increased [Ca(2+)](i) by TRPV1 activation was extruded by NCXs. Taken together, 
      this suggests that cAMP-mediated CB1-TRPV1 crosstalk and TRPV1-NCX coupling play 
      an important role in driving cellular functions following transduction of 
      external stimuli to odontoblasts.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Tsumura, Maki
AU  - Tsumura M
AD  - Oral Health Science Center hrc8, Tokyo Dental College, Chiba, Japan.
FAU - Sobhan, Ubaidus
AU  - Sobhan U
FAU - Muramatsu, Takashi
AU  - Muramatsu T
FAU - Sato, Masaki
AU  - Sato M
FAU - Ichikawa, Hideki
AU  - Ichikawa H
FAU - Sahara, Yoshinori
AU  - Sahara Y
FAU - Tazaki, Masakazu
AU  - Tazaki M
FAU - Shibukawa, Yoshiyuki
AU  - Shibukawa Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120530
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (2-(2-(4-(4-nitrobenzyloxy)phenyl)ethyl)isothiourea methanesulfonate)
RN  - 0 (Arachidonic Acids)
RN  - 0 (Calcium Channel Agonists)
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Cannabinoid Receptor Antagonists)
RN  - 0 (Diterpenes)
RN  - 0 (Endocannabinoids)
RN  - 0 (Glycerides)
RN  - 0 (Polyunsaturated Alkamides)
RN  - 0 (Receptors, Cannabinoid)
RN  - 0 (Sodium-Calcium Exchanger)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (Trpv1 protein, rat)
RN  - 8D239QDW64 (glyceryl 2-arachidonate)
RN  - A5O6P1UL4I (resiniferatoxin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - GYV9AM2QAG (Thiourea)
RN  - LFW48MY844 (capsazepine)
RN  - S07O44R1ZM (Capsaicin)
RN  - SY7Q814VUP (Calcium)
RN  - UR5G69TJKH (anandamide)
SB  - IM
MH  - Animals
MH  - Arachidonic Acids/pharmacology
MH  - Calcium/metabolism
MH  - Calcium Channel Agonists/pharmacology
MH  - Calcium Channel Blockers/pharmacology
MH  - Calcium Signaling/drug effects
MH  - Cannabinoid Receptor Antagonists
MH  - Capsaicin/analogs & derivatives/pharmacology
MH  - Cyclic AMP/metabolism
MH  - Diterpenes/pharmacology
MH  - Endocannabinoids
MH  - Glycerides/pharmacology
MH  - Hydrogen-Ion Concentration
MH  - Odontoblasts/cytology/drug effects/*metabolism
MH  - Polyunsaturated Alkamides/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Cannabinoid/*metabolism
MH  - Sodium-Calcium Exchanger/*metabolism
MH  - TRPV Cation Channels/agonists/antagonists & inhibitors/*metabolism
MH  - Thiourea/analogs & derivatives/pharmacology
EDAT- 2012/06/05 06:00
MHDA- 2012/10/31 06:00
CRDT- 2012/06/05 06:00
PHST- 2011/12/30 00:00 [received]
PHST- 2012/04/24 00:00 [revised]
PHST- 2012/05/02 00:00 [accepted]
PHST- 2012/06/05 06:00 [entrez]
PHST- 2012/06/05 06:00 [pubmed]
PHST- 2012/10/31 06:00 [medline]
AID - S0143-4160(12)00089-9 [pii]
AID - 10.1016/j.ceca.2012.05.002 [doi]
PST - ppublish
SO  - Cell Calcium. 2012 Aug;52(2):124-36. doi: 10.1016/j.ceca.2012.05.002. Epub 2012 
      May 30.