PMID- 22658782
OWN - NLM
STAT- MEDLINE
DCOM- 20121109
LR  - 20120817
IS  - 1879-3304 (Electronic)
IS  - 0039-6257 (Linking)
VI  - 57
IP  - 5
DP  - 2012 Sep
TI  - Monosomy 3 by FISH in uveal melanoma: variability in techniques and results.
PG  - 463-73
LID - 10.1016/j.survophthal.2011.12.004 [doi]
AB  - Tumor monosomy 3 confers a poor prognosis in patients with uveal melanoma. We 
      critically review the techniques used for fluorescence in situ hybridization 
      (FISH) detection of monosomy 3 in order to assess variability in practice 
      patterns and to explain differences in results. Significant variability that has 
      likely affected reported results was found in tissue sampling methods, selection 
      of FISH probes, number of cells counted, and the cut-off point used to determine 
      monosomy 3 status. Clinical parameters and specific techniques employed to report 
      FISH results should be specified so as to allow meta-analysis of published 
      studies. FISH-based detection of monosomy 3 in uveal melanoma has not been 
      performed in a standardized manner, which limits conclusions regarding its 
      clinical utility. FISH is a widely available, versatile technology, and when 
      performed optimally has the potential to be a valuable tool for determining the 
      prognosis of uveal melanoma.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Aronow, Mary
AU  - Aronow M
AD  - Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.
FAU - Sun, Yang
AU  - Sun Y
FAU - Saunthararajah, Yogen
AU  - Saunthararajah Y
FAU - Biscotti, Charles
AU  - Biscotti C
FAU - Tubbs, Raymond
AU  - Tubbs R
FAU - Triozzi, Pierre
AU  - Triozzi P
FAU - Singh, Arun D
AU  - Singh AD
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20120602
PL  - United States
TA  - Surv Ophthalmol
JT  - Survey of ophthalmology
JID - 0404551
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - DNA Probes
MH  - DNA, Neoplasm/analysis
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Melanoma/*genetics
MH  - Monosomy/*genetics
MH  - Uveal Neoplasms/*genetics
EDAT- 2012/06/05 06:00
MHDA- 2012/11/10 06:00
CRDT- 2012/06/05 06:00
PHST- 2011/04/29 00:00 [received]
PHST- 2011/12/21 00:00 [revised]
PHST- 2011/12/27 00:00 [accepted]
PHST- 2012/06/05 06:00 [entrez]
PHST- 2012/06/05 06:00 [pubmed]
PHST- 2012/11/10 06:00 [medline]
AID - S0039-6257(12)00006-9 [pii]
AID - 10.1016/j.survophthal.2011.12.004 [doi]
PST - ppublish
SO  - Surv Ophthalmol. 2012 Sep;57(5):463-73. doi: 10.1016/j.survophthal.2011.12.004. 
      Epub 2012 Jun 2.