PMID- 22658799
OWN - NLM
STAT- MEDLINE
DCOM- 20121022
LR  - 20131121
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 33
IP  - 26
DP  - 2012 Sep
TI  - A long-lasting dendritic cell DNA vaccination system using lysinylated 
      amphiphiles with mannose-mimicking head-groups.
PG  - 6220-9
LID - 10.1016/j.biomaterials.2012.05.006 [doi]
AB  - Dendritic cells (DCs) pulsed/transduced with tumor-associated or viral antigens 
      have shown promise in combating cancer and infectious diseases. Despite 
      significant progresses, development of a biologically safe DC-based genetic 
      immunization (DNA vaccination) system capable of providing truly long-lasting 
      protective immunity remains a significant scientific challenge. Here we show that 
      immunization with autologous DCs pre-transfected with electrostatic complexes 
      (lipoplexes) of a plasmid DNA encoding melanoma tumor associated antigen and 
      liposomes of two lysinylated cationic amphiphiles with mannose-mimicking quinic 
      and shikimic acid head-groups provides long-lasting (300 days post tumor 
      challenge) protective immunity with significant memory response (more than six 
      months after the second tumor challenge) in more than 80% immunized mice. The 
      presently described non-viral ex vivo DC-transfection system may be exploited in 
      inducing long-lasting immune response in DC-based genetic immunization.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Srinivas, Ramishetti
AU  - Srinivas R
AD  - Division of Lipid Science and Technology, Indian Institute of Chemical 
      Technology, Hyderabad 500 007, India.
FAU - Garu, Arup
AU  - Garu A
FAU - Moku, Gopikrishna
AU  - Moku G
FAU - Agawane, Sachin B
AU  - Agawane SB
FAU - Chaudhuri, Arabinda
AU  - Chaudhuri A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120531
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Vaccines, DNA)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - PHA4727WTP (Mannose)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/immunology/metabolism
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/metabolism
MH  - Flow Cytometry
MH  - Interferon-gamma/metabolism
MH  - Interleukin-4/metabolism
MH  - Male
MH  - Mannose/*chemistry
MH  - Melanoma/*immunology/metabolism/prevention & control
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Transfection/*methods
MH  - Vaccines, DNA/*chemistry/*therapeutic use
EDAT- 2012/06/05 06:00
MHDA- 2012/10/23 06:00
CRDT- 2012/06/05 06:00
PHST- 2012/03/26 00:00 [received]
PHST- 2012/05/04 00:00 [accepted]
PHST- 2012/06/05 06:00 [entrez]
PHST- 2012/06/05 06:00 [pubmed]
PHST- 2012/10/23 06:00 [medline]
AID - S0142-9612(12)00523-6 [pii]
AID - 10.1016/j.biomaterials.2012.05.006 [doi]
PST - ppublish
SO  - Biomaterials. 2012 Sep;33(26):6220-9. doi: 10.1016/j.biomaterials.2012.05.006. 
      Epub 2012 May 31.