PMID- 22659065
OWN - NLM
STAT- MEDLINE
DCOM- 20121128
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 185
IP  - 1
DP  - 2012 Oct
TI  - Development and evaluation of a novel reverse transcription loop-mediated 
      isothermal amplification (RT-LAMP) assay for detection of type II porcine 
      reproductive and respiratory syndrome virus.
PG  - 18-23
LID - 10.1016/j.jviromet.2012.05.016 [doi]
AB  - The objective of this study was to develop a reverse transcription loop-mediated 
      isothermal amplification (RT-LAMP) assay for detection of type II porcine 
      reproductive and respiratory syndrome virus (PRRSV). Based on sequence alignment, 
      four primers were designed amplifying the M gene of type II PRRSV and were 
      subsequently utilized in an RT-LAMP assay. The RT-LAMP product had a ladder-like 
      pattern of bands and the optimal reaction condition for this assay was determined 
      to be 40 min at 63 degrees C. Comparative analysis indicated that the RT-LAMP method was 
      more sensitive than a conventional RT-PCR assay and comparable to a real-time PCR 
      assay. In addition, the RT-LAMP assay was capable of detecting type II PRRSV in 
      field samples and differentiating type II PRRSV from seven other porcine viruses 
      which are all associated frequently with similar clinical symptoms.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Gao, Ming
AU  - Gao M
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 
      150030, China.
FAU - Cui, Jin
AU  - Cui J
FAU - Ren, Yudong
AU  - Ren Y
FAU - Suo, Siqingaowa
AU  - Suo S
FAU - Li, Guangxing
AU  - Li G
FAU - Sun, Xuejiao
AU  - Sun X
FAU - Su, Dingding
AU  - Su D
FAU - Opriessnig, Tanja
AU  - Opriessnig T
FAU - Ren, Xiaofeng
AU  - Ren X
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120529
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - DNA Primers/genetics
MH  - Genes, Viral
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/virology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Veterinary Medicine/*methods
MH  - Virology/*methods
EDAT- 2012/06/05 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/06/05 06:00
PHST- 2011/10/18 00:00 [received]
PHST- 2012/05/02 00:00 [revised]
PHST- 2012/05/21 00:00 [accepted]
PHST- 2012/06/05 06:00 [entrez]
PHST- 2012/06/05 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - S0166-0934(12)00179-6 [pii]
AID - 10.1016/j.jviromet.2012.05.016 [doi]
PST - ppublish
SO  - J Virol Methods. 2012 Oct;185(1):18-23. doi: 10.1016/j.jviromet.2012.05.016. Epub 
      2012 May 29.