PMID- 22659671
OWN - NLM
STAT- MEDLINE
DCOM- 20131219
LR  - 20181201
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 37
IP  - 5
DP  - 2012 May
TI  - [Transfection of BDNF gene promotes bone mesenchymal stem cells to differentiate 
      into neuron-like cells].
PG  - 441-6
LID - 10.3969/j.issn.1672-7347.2012.05.002 [doi]
AB  - OBJECTIVE: To provide further foundation for the therapy for spine cord injury by 
      transferred mesenchymal stem cells (MSC) expressing the brain-derived 
      neurotrophic factor (BDNF) gene. METHODS: Bone mesenchymal stem cells were 
      isolated from rats and directly cultivated and expanded in vitro. Bone 
      mesenchymal stem cells were divided into 3 groups: a pEGFP-N1- BDNF-transfected 
      group (BDNF group), a pEGFP-N1 transfected group (vector group) and a 
      non-transfected group. Western blot was applied to detect the expression of 
      transgenic bone mesenchymal stem cells. The differentiation of bone mesenchymal 
      stem cells was identified by immunofluorescence. The rates of induction of 
      neuron-like cells among the three cell groups were compared. RESULTS: The cell 
      surface markers of bone mesenchymal stem cells included expression of CD90 (+), 
      CD44 (+), CD34 (-), CD45 (-). The expression of BDNF in the transfected bone 
      mesenchymal stem cells was demonstrated by Western blot. The positive ratio of 
      neuron-like differentiation in the BDNF group was higher than that of both the 
      vector group and the non-transfected group (P<0.05). CONCLUSION: BDNF gene plays 
      an important role in promoting bone marrow mesenchymal stem cells to 
      differentiate into neuron-like cells.
FAU - Liu, Weidong
AU  - Liu W
AD  - Department of Spine Surgery, Second Xiangya Hospital, Central South University, 
      Changsha, China.
FAU - Lu, Guohua
AU  - Lu G
FAU - Wang, Bing
AU  - Wang B
FAU - Ma, Zemin
AU  - Ma Z
FAU - Li, Yawei
AU  - Li Y
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cell Differentiation/*genetics
MH  - Cells, Cultured
MH  - Female
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology
MH  - Neurons/*cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transfection
EDAT- 2012/06/05 06:00
MHDA- 2013/12/20 06:00
CRDT- 2012/06/05 06:00
PHST- 2012/06/05 06:00 [entrez]
PHST- 2012/06/05 06:00 [pubmed]
PHST- 2013/12/20 06:00 [medline]
AID - 10.3969/j.issn.1672-7347.2012.05.002 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012 May;37(5):441-6. doi: 
      10.3969/j.issn.1672-7347.2012.05.002.