PMID- 22660740
OWN - NLM
STAT- MEDLINE
DCOM- 20120917
LR  - 20211021
IS  - 1548-7105 (Electronic)
IS  - 1548-7091 (Print)
IS  - 1548-7091 (Linking)
VI  - 9
IP  - 7
DP  - 2012 Jun 3
TI  - Single-cell systems biology by super-resolution imaging and combinatorial 
      labeling.
PG  - 743-8
LID - 10.1038/nmeth.2069 [doi]
AB  - Fluorescence microscopy is a powerful quantitative tool for exploring regulatory 
      networks in single cells. However, the number of molecular species that can be 
      measured simultaneously is limited by the spectral overlap between fluorophores. 
      Here we demonstrate a simple but general strategy to drastically increase the 
      capacity for multiplex detection of molecules in single cells by using optical 
      super-resolution microscopy (SRM) and combinatorial labeling. As a proof of 
      principle, we labeled mRNAs with unique combinations of fluorophores using 
      fluorescence in situ hybridization (FISH), and resolved the sequences and 
      combinations of fluorophores with SRM. We measured mRNA levels of 32 genes 
      simultaneously in single Saccharomyces cerevisiae cells. These experiments 
      demonstrate that combinatorial labeling and super-resolution imaging of single 
      cells is a natural approach to bring systems biology into single cells.
FAU - Lubeck, Eric
AU  - Lubeck E
AD  - Program in Biochemistry and Molecular Biophysics, California Institute of 
      Technology, Pasadena, California, USA.
FAU - Cai, Long
AU  - Cai L
LA  - eng
GR  - DP2 OD008530/OD/NIH HHS/United States
GR  - R01 HD075605/HD/NICHD NIH HHS/United States
GR  - 1DP2OD008530/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120603
PL  - United States
TA  - Nat Methods
JT  - Nature methods
JID - 101215604
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Messenger)
SB  - IM
CIN - Nat Methods. 2014 Apr;11(4):360-1. PMID: 24681720
MH  - Fluorescent Dyes
MH  - Gene Expression Profiling
MH  - Image Processing, Computer-Assisted/instrumentation/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence/instrumentation/*methods
MH  - RNA, Messenger/genetics
MH  - Saccharomyces cerevisiae/genetics
MH  - Single-Cell Analysis/instrumentation/*methods
MH  - Systems Biology/instrumentation/*methods
PMC - PMC3418883
MID - NIHMS397912
EDAT- 2012/06/05 06:00
MHDA- 2012/09/18 06:00
PMCR- 2013/01/01
CRDT- 2012/06/05 06:00
PHST- 2012/03/02 00:00 [received]
PHST- 2012/05/07 00:00 [accepted]
PHST- 2012/06/05 06:00 [entrez]
PHST- 2012/06/05 06:00 [pubmed]
PHST- 2012/09/18 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - nmeth.2069 [pii]
AID - 10.1038/nmeth.2069 [doi]
PST - epublish
SO  - Nat Methods. 2012 Jun 3;9(7):743-8. doi: 10.1038/nmeth.2069.