PMID- 22661432
OWN - NLM
STAT- MEDLINE
DCOM- 20120919
LR  - 20121001
IS  - 1559-6095 (Electronic)
IS  - 1559-6095 (Linking)
VI  - 2012
IP  - 6
DP  - 2012 Jun 1
TI  - Rescue of S/MAR-containing nonviral episomal expression vectors.
PG  - 709-11
LID - 10.1101/pdb.prot069518 [doi]
AB  - The episomal status of S/MAR (scaffold/matrix attached region)-based vectors can 
      be confirmed by several methods including Southern blots, fluorescence in situ 
      hybridization (FISH) analysis, or plasmid rescue experiments. In rescue 
      experiments, genomic DNA (gDNA) or DNA from Hirt extracts is isolated from cell 
      clones or mixed populations in which S/MAR plasmids are stably established. 
      Bacteria are transformed with this DNA and if episomal plasmid DNA (pDNA) is 
      present, resistant bacterial colonies will form.
FAU - Hagedorn, Claudia
AU  - Hagedorn C
AD  - Centre for Biomedical Education and Research, Institute of Cell Biology, 
      University of Witten/Herdecke, 58453 Witten, Germany.
FAU - Baiker, Armin
AU  - Baiker A
FAU - Postberg, Jan
AU  - Postberg J
FAU - Ehrhardt, Anja
AU  - Ehrhardt A
FAU - Lipps, Hans J
AU  - Lipps HJ
LA  - eng
PT  - Journal Article
DEP - 20120601
PL  - United States
TA  - Cold Spring Harb Protoc
JT  - Cold Spring Harbor protocols
JID - 101524530
SB  - IM
MH  - Escherichia coli/genetics
MH  - Genetic Engineering/*methods
MH  - Genetic Vectors/*analysis/*isolation & purification
MH  - Plasmids/*analysis/*isolation & purification
MH  - *Transformation, Bacterial
MH  - *Transformation, Genetic
EDAT- 2012/06/05 06:00
MHDA- 2012/09/20 06:00
CRDT- 2012/06/05 06:00
PHST- 2012/06/05 06:00 [entrez]
PHST- 2012/06/05 06:00 [pubmed]
PHST- 2012/09/20 06:00 [medline]
AID - 2012/6/pdb.prot069518 [pii]
AID - 10.1101/pdb.prot069518 [doi]
PST - epublish
SO  - Cold Spring Harb Protoc. 2012 Jun 1;2012(6):709-11. doi: 10.1101/pdb.prot069518.