PMID- 22661439
OWN - NLM
STAT- MEDLINE
DCOM- 20120919
LR  - 20220318
IS  - 1559-6095 (Electronic)
IS  - 1559-6095 (Linking)
VI  - 2012
IP  - 6
DP  - 2012 Jun 1
TI  - Imaging neuronal activity with genetically encoded calcium indicators.
PG  - 647-56
LID - 10.1101/pdb.top069609 [doi]
AB  - Genetically encoded calcium indicators (GECIs), which are based on chimeric 
      fluorescent proteins, can be used to monitor calcium transients in living cells 
      and organisms. Because they are encoded by DNA, GECIs can be delivered to the 
      intact brain noninvasively and targeted to defined populations of neurons and 
      specific subcellular compartments for long-term, repeated measurements in vivo. 
      GECIs have improved iteratively and are becoming useful for imaging neural 
      activity in vivo. Here we summarize extrinsic and intrinsic factors that 
      influence a GECI's performance and provides guidelines for selecting the 
      appropriate GECI for a given application. We also review recent progress in GECI 
      design, optimization, and standardized testing protocols.
FAU - Tian, Lin
AU  - Tian L
FAU - Hires, S Andrew
AU  - Hires SA
FAU - Looger, Loren L
AU  - Looger LL
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20120601
PL  - United States
TA  - Cold Spring Harb Protoc
JT  - Cold Spring Harbor protocols
JID - 101524530
RN  - 0 (Luminescent Proteins)
RN  - 0 (Recombinant Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*analysis
MH  - Genes, Reporter
MH  - Image Processing, Computer-Assisted/*methods
MH  - Luminescent Proteins/genetics/metabolism
MH  - Microscopy, Fluorescence/*methods
MH  - Neurons/chemistry/*physiology
MH  - Recombinant Proteins/genetics/metabolism
EDAT- 2012/06/05 06:00
MHDA- 2012/09/20 06:00
CRDT- 2012/06/05 06:00
PHST- 2012/06/05 06:00 [entrez]
PHST- 2012/06/05 06:00 [pubmed]
PHST- 2012/09/20 06:00 [medline]
AID - 2012/6/pdb.top069609 [pii]
AID - 10.1101/pdb.top069609 [doi]
PST - epublish
SO  - Cold Spring Harb Protoc. 2012 Jun 1;2012(6):647-56. doi: 10.1101/pdb.top069609.