PMID- 22663932
OWN - NLM
STAT- MEDLINE
DCOM- 20121129
LR  - 20120717
IS  - 1743-1328 (Electronic)
IS  - 0161-6412 (Linking)
VI  - 34
IP  - 6
DP  - 2012 Jul
TI  - A rapid, novel model of culturing cranial nerve X-derived motoneurons for 
      screening trophic factor outgrowth response.
PG  - 564-75
LID - 10.1179/1743132812Y.0000000046 [doi]
AB  - OBJECTIVES: After cranial nerve X (CN X) injury, vocal fold paralysis treatments 
      currently face a myriad of obstacles in achieving non-synkinetic, functional 
      reinnervation. Of particular therapeutic interest is the targeted administration 
      of locally expressed biological neurotrophic factors (NFs). To date, a method to 
      culture mature CN X motoneurons for NF responsiveness screening has not been 
      described. METHODS: We herein present a novel method for establishing mature 
      murine CN X motoneuron cultures, and use the model to test CN X motoneuron 
      outgrowth response to individual and paired ascending concentrations of selected 
      neurotrophic factors [glial cell-derived neurotrophic factor (GDNF), 
      brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor 
      (CNTF)]. RESULTS: Findings demonstrated low concentration (5 ng/ml) CNTF to have 
      the greatest positive effect on motoneuron outgrowth, beyond that of both 
      indivual NF and paired NF combinations, based on total neurite outgrowth [mean 
      total neurite outgrowth = 445.7+/-84.45 mum in the (5 ng/ml) CNTF group versus 
      179.7+/-13.63 mum in saline controls (P<0.01)]. Paired treatments with CNTF/GDNF, 
      and CNTF/BDNF promoted motoneuron branching at a variety of concentrations beyond 
      saline controls, and paired GDNF/BDNF had inhibitory effects on motoneuron 
      branching. DISCUSSION: Our described in vitro model of establishing mature CN X 
      cultures allowed rapid screening for responsiveness to therapeutic NFs at a 
      variety of concentrations and combinations. While the model ultimately may be 
      used to investigate the molecular mechanisms of CN X motoneuron regeneration, the 
      current study identified CNTF as a promising therapeutic candidate for the 
      promotion of CN X outgrowth.
FAU - McRae, Bryan R
AU  - McRae BR
AD  - Department of Otolaryngology-Head and Neck Surgery, Indiana University School of 
      Medicine, Indianapolis, IN 46202, USA.
FAU - Shew, Matthew
AU  - Shew M
FAU - Aaron, Geoffrey P
AU  - Aaron GP
FAU - Bijangi-Vishehsaraei, Khadijeh
AU  - Bijangi-Vishehsaraei K
FAU - Halum, Stacey L
AU  - Halum SL
LA  - eng
GR  - K08DC009583/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120530
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/*methods
MH  - Fluorescent Antibody Technique
MH  - Motor Neurons/*cytology/drug effects
MH  - Nerve Growth Factors/*metabolism/pharmacology
MH  - Neurites/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Vagus Nerve/*cytology/drug effects
EDAT- 2012/06/06 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/06/06 06:00
PHST- 2012/06/06 06:00 [entrez]
PHST- 2012/06/06 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - ner2714 [pii]
AID - 10.1179/1743132812Y.0000000046 [doi]
PST - ppublish
SO  - Neurol Res. 2012 Jul;34(6):564-75. doi: 10.1179/1743132812Y.0000000046. Epub 2012 
      May 30.