PMID- 22664543
OWN - NLM
STAT- MEDLINE
DCOM- 20120830
LR  - 20211021
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 63
DP  - 2012 May 25
TI  - Use of human perivascular stem cells for bone regeneration.
PG  - e2952
LID - 2952 [pii]
LID - 10.3791/2952 [doi]
AB  - Human perivascular stem cells (PSCs) can be isolated in sufficient numbers from 
      multiple tissues for purposes of skeletal tissue engineering. PSCs are a 
      FACS-sorted population of 'pericytes' (CD146+CD34-CD45-) and 'adventitial cells' 
      (CD146-CD34+CD45-), each of which we have previously reported to have properties 
      of mesenchymal stem cells. PSCs, like MSCs, are able to undergo osteogenic 
      differentiation, as well as secrete pro-osteogenic cytokines. In the present 
      protocol, we demonstrate the osteogenicity of PSCs in several animal models 
      including a muscle pouch implantation in SCID (severe combined immunodeficient) 
      mice, a SCID mouse calvarial defect and a femoral segmental defect (FSD) in 
      athymic rats. The thigh muscle pouch model is used to assess ectopic bone 
      formation. Calvarial defects are centered on the parietal bone and are standardly 
      4 mm in diameter (critically sized). FSDs are bicortical and are stabilized with 
      a polyethylene bar and K-wires. The FSD described is also a critical size defect, 
      which does not significantly heal on its own. In contrast, if stem cells or 
      growth factors are added to the defect site, significant bone regeneration can be 
      appreciated. The overall goal of PSC xenografting is to demonstrate the 
      osteogenic capability of this cell type in both ectopic and orthotopic bone 
      regeneration models.
FAU - James, Aaron W
AU  - James AW
AD  - Dental and Craniofacial Research Institute and Section of Orthodontics, School of 
      Dentistry, UCLA, USA.
FAU - Zara, Janette N
AU  - Zara JN
FAU - Corselli, Mirko
AU  - Corselli M
FAU - Chiang, Michael
AU  - Chiang M
FAU - Yuan, Wei
AU  - Yuan W
FAU - Nguyen, Virginia
AU  - Nguyen V
FAU - Askarinam, Asal
AU  - Askarinam A
FAU - Goyal, Raghav
AU  - Goyal R
FAU - Siu, Ronald K
AU  - Siu RK
FAU - Scott, Victoria
AU  - Scott V
FAU - Lee, Min
AU  - Lee M
FAU - Ting, Kang
AU  - Ting K
FAU - Peault, Bruno
AU  - Peault B
FAU - Soo, Chia
AU  - Soo C
LA  - eng
GR  - T32 DE007296/DE/NIDCR NIH HHS/United States
GR  - G1000816/MRC_/Medical Research Council/United Kingdom
GR  - R01 DE01607/DE/NIDCR NIH HHS/United States
GR  - 5T32DE007296-14/DE/NIDCR NIH HHS/United States
GR  - R21 DE0177711/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20120525
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - *Bone Regeneration
MH  - Femur/pathology
MH  - Humans
MH  - Mice
MH  - Mice, SCID
MH  - Models, Animal
MH  - Pericytes/*cytology
MH  - Rats
MH  - Rats, Nude
MH  - Skull/pathology
MH  - Stem Cells/*cytology
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds
PMC - PMC3466949
EDAT- 2012/06/06 06:00
MHDA- 2012/08/31 06:00
PMCR- 2012/05/25
CRDT- 2012/06/06 06:00
PHST- 2012/06/06 06:00 [entrez]
PHST- 2012/06/06 06:00 [pubmed]
PHST- 2012/08/31 06:00 [medline]
PHST- 2012/05/25 00:00 [pmc-release]
AID - 2952 [pii]
AID - 10.3791/2952 [doi]
PST - epublish
SO  - J Vis Exp. 2012 May 25;(63):e2952. doi: 10.3791/2952.