PMID- 22665226 OWN - NLM STAT- MEDLINE DCOM- 20121015 LR - 20120605 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 882 DP - 2012 TI - HLA typing by SSO and SSP methods. PG - 9-25 LID - 10.1007/978-1-61779-842-9_2 [doi] AB - Human leukocyte antigen (HLA) typing, utilising the sequence-specific oligonucleotide (SSO) and sequence-specific primer (SSP) technologies, has been in routine use in many tissue typing laboratories worldwide for more than 20 years since the development of the polymerase chain reaction. Both methods are very useful for clinical and research purposes and can provide generic (low resolution) to allelic (high resolution) typing results. This chapter provides an overview of the SSO and SSP methods in relation to HLA typing. FAU - Dunckley, Heather AU - Dunckley H AD - Australian Red Cross Blood Service, Sydney, NSW, Australia. hdunckley@optusnet.com.au LA - eng PT - Journal Article PT - Review PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 0 (DNA Primers) RN - 0 (Oligonucleotides) SB - IM MH - Base Sequence MH - DNA Primers/*genetics MH - Histocompatibility Testing/*methods MH - Humans MH - Oligonucleotides/*genetics MH - Polymerase Chain Reaction/methods EDAT- 2012/06/06 06:00 MHDA- 2012/10/16 06:00 CRDT- 2012/06/06 06:00 PHST- 2012/06/06 06:00 [entrez] PHST- 2012/06/06 06:00 [pubmed] PHST- 2012/10/16 06:00 [medline] AID - 10.1007/978-1-61779-842-9_2 [doi] PST - ppublish SO - Methods Mol Biol. 2012;882:9-25. doi: 10.1007/978-1-61779-842-9_2.